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January 21st, 2025
Version: 2
University of California, San Diego
molecular biology
biorxiv

Description of a novel extremophile green algae, Chlamydomonas pacifica, and its potential as a biotechnology host

Dutra Molino, J. V.Open in Google Scholar•Oliver, A.Open in Google Scholar•Sethuram, H.Open in Google Scholar•Kang, K.Open in Google Scholar•Saucedo, B.Open in Google Scholar•Diaz, C. J.Open in Google Scholar•Gupta, A.Open in Google Scholar•Jen, L. J.Open in Google Scholar•Torres-tiji, Y.Open in Google Scholar•Hidasi, N.Open in Google Scholaret al.

We present the comprehensive characterization of a newly identified microalga, Chlamydomonas pacifica, originally isolated from a soil sample in San Diego, CA, USA. This species showcases remarkable biological versatility, including a broad pH range tolerance (6-11.5), high thermal tolerance (up to 42 degree celsius), and salinity resilience (up to 2% NaCl). Its amenability to genetic manipulation and sexual reproduction via mating, particularly between the two opposing strains CC-5697 & CC-5699, now publicly available through the Chlamydomonas Resource Center, underscores its potential as a biotechnological chassis. The biological assessment of C. pacifica revealed versatile metabolic capabilities, including diverse nitrogen assimilation capability, motility and phototaxis. Genomic and transcriptomic analyses identified 17,829 genes within a 121 Mb genome, featuring a GC content of 61%. The codon usage of C. pacifica closely mirrors that of C. reinhardtii, indicating a conserved genetic architecture that supports a trend in codon preference with minor variations. Phylogenetic analyses position C. pacifica within the core-Reinhardtinia clade yet distinct from known Volvocales species. The lipidomic data revealed an abundance of triacylglycerols (TAGs), promising for biofuel applications and lipids for health-related benefits. Our investigation lays the groundwork for exploiting C. pacifica in biotechnological applications, from biofuel generation to synthesizing biodegradable plastics, positioning it as a versatile host for future bioengineering endeavors.

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