2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
January 21st, 2025
Version: 1
Meharry Medical College
genomics
biorxiv

Intersecting Transcriptomic Landscapes of Hypertension and Kidney Function in African American Women

Abbas, M.Open in Google Scholar•Martin, P.Open in Google Scholar•Lindsey, M.Open in Google Scholar•Bennett, E. S.Open in Google Scholar•Brown, T. L.Open in Google Scholar•Nzerue, C.Open in Google Scholar•Williams, C. R.Open in Google Scholar•Gaye, A.Open in Google Scholar

Background: Hypertension is a major risk factor for chronic kidney disease (CKD) and disproportionately affects African American women, contributing to disparities in kidney health outcomes. The biological mechanisms connecting hypertension to reduced kidney function, particularly in understudied populations, remain poorly understood. This study leverages transcriptomic analyses to uncover shared molecular signatures associated with hypertension and kidney function, focusing on female-specific profiles. Methods: The study analyzed whole-blood mRNA sequencing data from a cohort of 344 African American women, divided equally into discovery (n = 172) and validation (n = 172) datasets, along with 147 African American men. Differential expression (DE) analyses were performed to identify mRNAs associated with hypertension and kidney function (measured as eGFR). Female-specific findings were determined by comparing results between females and males. Pathway enrichment analyses were subsequently conducted to link the identified mRNAs to key biological mechanisms. Results: Comparative analyses revealed unique transcriptomic profiles in females, underscoring the role of sex-specific factors in disease progression. DE analyses identified 95 female-specific genes associated with both hypertension and eGFR. Subsequent pathway enrichment analysis with the 95 genes revealed key pathways related to fibrosis, inflammation, lipid metabolism, and endothelial dysfunction. The list of 95 includes TGF-{beta}1 and PNPLA2 implicated in fibrotic and metabolic dysregulation, and immune system players such as IL32 and TNFSF12 that amplify inflammation and kidney injury. Conclusions: This study provides novel insights into the transcriptomic mechanisms underlying hypertension and kidney function in African American women. The findings emphasize the importance of addressing sex-specific and population-specific molecular mechanisms to inform precision medicine approaches and reduce health disparities hypertension-related impaired kidney function. Future research should prioritize experimental validation and longitudinal studies to further elucidate these pathways.

Similar Papers

biorxiv
Wed Jan 22 2025
Draft genome sequences of four lactic acid bacteria from fermented chicken meat unveil biosynthetic gene clusters for antimicrobial compounds
Lactic acid bacteria play a crucial role in fermented food production and serve as important sources of antimicrobial peptides. This study reports four lactic acid bacteria strains, isolated from fermented chicken meat, which harbor biosynthetic gene clusters encoding antimicrobial compounds. These strains are classified within the genera Pediococcus and Lactiplantibacillus.
Alam, M.
•
Hassan, T.
•
Nizami, T. A.
•
Akter, L.
•
Rakib, T. M.
biorxiv
Wed Jan 22 2025
Epigenetic mechanisms regulating CD8+ T cell senescence in aging humans
Aging leads to the decline of immunity, rendering the elderly susceptible to infection and disease. In the CD8+ T cell compartment, aging leads to a substantial increase of cells with high levels of senescence-associated beta-galactosidase activity (SA-BGal) and other senescence characteristics, including a pro-inflammatory transcriptome and impaired proliferative potential. Using senescent cell i...
Turano, P. S.
•
Dewald, H. K.
•
Akbulut, E.
•
Vasilopoulos, T.
...•
Martinez Zamudio, R. I.
biorxiv
Wed Jan 22 2025
Pervasive and programmed nucleosome distortion patterns on single mammalian chromatin fibers
We present a genome-scale method to map the single-molecule co-occupancy of structurally distinct nucleosomes, subnucleosomes, and other protein-DNA interactions via long-read high-resolution adenine methyltransferase footprinting. Iteratively Defined Lengths of Inaccessibility (IDLI) classifies nucleosomes on the basis of shared patterns of intranucleosomal accessibility, into: i.) minimally-acce...
Yang, M. G.
•
Richter, H. J.
•
Wang, S.
•
McNally, C. P.
...•
Ramani, V.
biorxiv
Wed Jan 22 2025
Low level contamination confounds population genomic analysis
Genome sequence contamination has a variety of causes and can originate from within or between species. Previous research focused primarily on cross-species contamination or on prokaryotes. This paper visualizes B-allele frequency to test for intra-species contamination, and measures its effects on phylogenetic and admixture analysis in two fungal species. Using a standard base calling pipeline, w...
Ward, A.
•
Scopel, E. F. C.
•
Shuman, B.
•
Momany, M.
•
Bensasson, D.
biorxiv
Wed Jan 22 2025
Genome Sequencing, Molecular Marker Development and Genetic Diversity Assessment of Economically Important Vulnerable Tree Species Saraca asoca (Roxb.) W.J de Wilde
Saraca asoca, is an understory tree along streams in evergreen to semi-evergreen forests up to 600 m. It is an important tree in cultural tradition and medicinally significant. It is native to India and Sri Lanka. Globally the species is found to occur in India, Sri Lanka, Myanmar, Bangladesh. It was introduced in Malaysia. Within India, it\'s found in Western Ghat and Eastern Ghat. It is occasion...
P, M.
•
Hegde, S. N.
•
Gowda, M.
•
Kareem, A.
...•
Narendran, P.
biorxiv
Wed Jan 22 2025
Single-cell decoding of human islet cell type-specific alterations in type 2 diabetes reveals converging genetic- and state-driven β-cell gene expression defects
Pancreatic islets maintain glucose homeostasis through coordinated action of their constituent endocrine and affiliate cell types and are central to type 2 diabetes (T2D) genetics and pathophysiology. Our understanding of robust human islet cell type-specific alterations in T2D remains limited. Here, we report comprehensive single cell transcriptome profiling of 245,878 human islet cells from a 48...
Bandesh, K.
•
Motakis, E.
•
Nargund, S.
•
Kursawe, R.
...•
Stitzel, M. L.
biorxiv
Wed Jan 22 2025
Mouse Single-Cell Long-Read Splicing Atlas by Ouro-Seq
Alternative splicing creates cellular and phenotypic diversity; thus, characterizing splicing patterns across diverse cell types is essential for a comprehensive understanding of these cell types. However, existing full-length single-cell RNA-sequencing methods suffer from multiple technical limitations, such as the pervasive presence of reverse transcription artifacts across genes. Here, we intro...
An, H.
•
Choi, S. Y.
•
Yi, J.
•
Lim, C.
...•
Park, J.
biorxiv
Wed Jan 22 2025
Comparative genomic analysis of a novel heat-tolerant and euryhaline strain of unicellular marine cyanobacterium Cyanobacterium sp. DS4 from a high-temperature lagoon
Background: Cyanobacteria have diversified through their long evolutionary history and occupy a wide range of environments on Earth. To advance our understanding of their adaption mechanisms in extreme environments, we performed stress tolerance characterizations, whole genome sequencing, and comparative genomic analyses of a novel heat-tolerant and euryhaline strain of the unicellular cyanobacter...
Chen, C.-N. N.
•
Lin, K.-M.
•
Lin, Y.-C.
•
Chang, H.-Y.
...•
Chu, H.-A.
biorxiv
Tue Jan 21 2025
A Single-Cell Atlas of the Upper Respiratory Epithelium Reveals Heterogeneity in Cell Types and Patterning Strategies
The upper respiratory tract, organized along the pharyngolaryngeal-to-tracheobronchial axis, is essential for homeostatic functions such as breathing and vocalization. The upper respiratory epithelium is frequently exposed to pollutants and pathogens, making this an area of first-line defense against respiratory injury and infection. The respiratory epithelium is composed of a rich array of specia...
Sun, X.
•
Foote, A.