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March 30th, 2025
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Agriculture and AgriFood Canada
genomics
biorxiv

Exploring the mycovirome: novel and diverse mycoviruses in Botrytis cinerea

Drury, S. C.Open in Google Scholar•Poursalavati, A.Open in Google Scholar•Lemoyne, P.Open in Google Scholar•Xu, D.Open in Google Scholar•Moffett, P.Open in Google Scholar•Carisse, O.Open in Google Scholar•Van der Heyden, H.Open in Google Scholar•Fall, M. L.Open in Google Scholar

Botrytis cinerea is a necrotrophic fungal pathogen that causes significant economic losses to many crops.The management of B. cinerea is difficult due to a rise in fungicide resistance. Harnessing mycoviruses that cause hypovirulence in B. cinerea is a promising alternative.This research explored, for the first time in Canada, the mycovirome of B. cinerea. Isolates of B. cinerea were collected from fruits and vegetables. Fitness and pathogenicity criteria, including sclerotia production, colony morphotype, and lesion size were evaluated. A double-stranded RNA (dsRNA) extraction was used to sequence dsRNA from 45 isolates with low fitness/pathogenicity, and the mycovirome was profiled. Mycoviruses were identified in 44/45 isolates. Most of these had positive single-stranded RNA or dsRNA genomes, and a small number had negative single-stranded RNA, single-stranded DNA, or reverse transcriptase RNA genomes. Following deep analysis of RNA-dependent RNA polymerase and replication initiation proteins, a total of 62 unique contigs were identified belonging to new strains of mycovirus species.Four putative novel mycovirus species belonging to Endornaviridae, Botybirnaviridae, Peribunyaviridae, and Bunyavirales taxa were identified.This study revealed a high degree of diversity in the mycovirome of B. cinerea. Species accumulation curve analysis indicated that, with the number of isolates characterized, we were unable to capture the full extent of expected diversity. Nevertheless, we identified potential hypovirulence-inducing mycoviruses, including Botrytis cinerea mitovirus 1, Botrytis cinerea hypovirus 1, and Botrytis porri botybirnavirus 1. Some of these novel mycoviruses belonged to taxa known to produce viral particles, which can be an interesting feature for their use as biocontrol agents.

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