Aquatic invertebrates are a diverse, nutrient-dense, and socio-ecologically important food whose contribution to human nutrition is frequently overlooked. We quantify their contribution to global nutrient supplies and estimate the nutrient content of >50,000 macroinvertebrate species. Current aquatic invertebrate production supplies the equivalent annual requirement for >6 billion people in terms of vitamin B12 and selenium; >1 billion people for copper, omega 3 fatty acids, iodine and zinc; and >100 million people for nutrients such as vitamins B2 and B3, iron, manganese, and magnesium. Nutrient composition differs among taxonomic groups, consumption patterns, and environmental and life-history factors. Our study highlights the benefits of integrating aquatic invertebrates into dietary portfolios across global societies, mainstreaming their nutritional importance in development projects, sustainability assessments and food policy.