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April 1st, 2025
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Texas State University
molecular biology
biorxiv

Pseudogymnoascus destructans transcriptional response to chronic copper stress

Anne, S.Open in Google Scholar•McDonald, M. R.Open in Google Scholar•Lu, Y.Open in Google Scholar•Peterson, R. L.Open in Google Scholar

Copper (Cu) is an essential metal micronutrient, and a fungal pathogens\' ability to thrive in diverse niches across a broad range of bioavailable copper levels is vital for host-colonization and fungal-propagation. Recent transcriptomic studies have implemented that trace metal acquisition is important for the propagation of the white nose syndrome (WNS) causing fungus, Pseudogymnoascus destructans, on bat hosts. This report characterizes the P. destructans transcriptional response to Cu withholding and Cu overload stress. We identify 583 differently expressed genes (DEGs) that respond to Cu withholding stress and 667 DEGs that respond to Cu overload stress. We find that the P. destructans Cu transporter genes CTR1a and CTR1b, as well as two homologs to Cryptococcus neoformans Cbi1/BIM1 VC83-03095 (BLP2) and VC83-07867 (BLP3) are highly regulated by Cu withholding stress. We identify a cluster of genes, VC83 01834 to VC83 01837, that are regulated by copper bioavailability, which we identify as the Cu Responsive gene Cluster (CRC). We find that chronic exposure to elevated copper levels leads to an increase in genes associated with DNA repair and DNA replication fidelity. A comparison of our transcriptomic data sets with P. destructans at WNS fungal infection sites reveals several putative fungal virulence factors that respond to environmental copper stress.

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