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April 3rd, 2025
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University of Florida
cancer biology
biorxiv

An LC-MS/MS method for the quantification of tobacco-specific carcinogen protein adducts.

Xing, C.Open in Google Scholar•Freeman, B. N.Open in Google Scholar

4-(Methyl-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and its major metabolite 4- (methylnitrosamino)-l-(3-pyridine)-l-butanol (NNAL) are tobacco-specific lung carcinogens. Methods have been developed to quantify NNK- and NNAL-specific DNA adducts in pre-clinical samples but are less feasible to translation due to limited access to target tissues for sufficient DNA. NNAL-specific DNA or protein adducts have never been detected in clinical samples, which are critical to support the physiological relevance of NNAL bioactivation and carcinogenesis. We reported a sensitive and specific LC-MS/MS method to quantify hydrolyzed NNAL adduct, 1-(3-pyridinyl)-1,4-butanediol (PBD). The method was applicable to variety of bio-logical samples, to assess tobacco exposure and estimate NNAL bioactivation.

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