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April 3rd, 2025
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Nara Institute of Science and Technology
molecular biology
biorxiv

Structural basis of a phosphotransferase system xylose transporter

Takahashi, Y. S.Open in Google Scholar•Kohga, H.Open in Google Scholar•Chek, M. F.Open in Google Scholar•Yamamoto, K.Open in Google Scholar•Takahashi, J. F.Open in Google Scholar•Shigematsu, H.Open in Google Scholar•Tanaka, Y.Open in Google Scholar•Ichikawa, M.Open in Google Scholar•Miyazaki, R.Open in Google Scholar•Hakoshima, T.Open in Google Scholaret al.

The bacterial phosphotransferase system (PTS) is essential for carbohydrate uptake, and IIC transporters in the system facilitate intracellular sugar transport. Here, we report the crystal and cryo-EM structures of Leminorella grimontii GatC (LgGatC), a putative xylose transporter in the IIC family, in its outward-facing state. Structural analysis indicates that the outward-facing cavity recognizes the linear form of D-xylose. A structure-based homology model of an inward-facing state supports an elevator-like sugar transport mechanism. These findings provide insights into PTS-mediated xylose transport and its potential applications in microbial engineering.

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