2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
April 6th, 2025
Version: 1
Henan Agricultural University
biochemistry
biorxiv

THA_1941 from Thermosipho africanus: A Thermostable β-1,3-Glucan Phosphorylase for Efficient β-1,3-Glucan Synthesis

Mao, G.Open in Google Scholar•Yu, J.Open in Google Scholar•Lin, J.Open in Google Scholar•Song, M.Open in Google Scholar•Su, Z.Open in Google Scholar•Xie, H.Open in Google Scholar•Zhang, H.Open in Google Scholar•Chen, H.Open in Google Scholar•Song, A.Open in Google Scholar

{beta}-1,3-Glucan phosphorylases capable of utilizing glucose as a priming substrate are key biocatalysts for the synthesis of functional {beta}-1,3-glucan. In this study, we identified THA_1941 from Thermosipho africanus (Ta{beta}GP) as a GH161 {beta}-1,3-glucan phosphorylase exhibiting robust synthetic activity towards glucose, as confirmed by 13C nuclear magnetic resonance, liquid chromatography-mass spectrometry, and sequence and structural analyses. Ta{beta}GP displayed exceptional thermostability, retaining 93% of its activity at 60 {degrees}C for 180 h, and showed broad pH tolerance ranging from pH 5.0 to 10.0, surpassing the performance of previously reported homologs. In addition, Ta{beta}GP exhibited broad substrate flexibility, accepting both - and {beta}-linked disaccharides, and demonstrated strong resistance to metal ions and lignocellulose-derived inhibitors. In the presence of 150 mM glucose 1-phosphate as the donor substrate, Ta{beta}GP synthesized {beta}-1,3-glucan with a tunable average degree of polymerization (10-32), depending on the concentration of glucose used as the primer. The combination of thermostability, inhibitor resistance, and substrate versatility makes Ta{beta}GP a promising biocatalyst for the economically viable and environmentally sustainable synthesis of {beta}-1,3-glucan from non-food biomass sources.

Similar Papers

biorxiv
Mon Apr 07 2025
Molecular basis for multidrug efflux by an anaerobic RND transporter
Bacteria can resist antibiotics and toxic substances within demanding ecological settings, such as low oxygen, extreme acid, and during nutrient starvation. MdtEF, a proton motive force-driven efflux pump from the resistance-nodulation-cell division (RND) superfamily, is upregulated in these conditions but its molecular mechanism is unknown. Here, we report cryo-electron microscopy structures of E...
Lawrence, R.
•
Athar, M.
•
Uddin, M. R.
•
Adams, C.
...•
Reading, E.
biorxiv
Mon Apr 07 2025
AQuaRef: Machine learning accelerated quantum refinement of protein structures
Cryo-EM and X-ray crystallography provide crucial experimental data for obtaining atomic-detail models of biomacromolecules. Refining these models relies on library-based stereochemical restraints, which, in addition to being limited to known chemical entities, do not include meaningful noncovalent interactions relying solely on nonbonded repulsions. Quantum mechanical (QM) calculations could alle...
Zubatyuk, R.
•
Biczysko, M.
•
Ranasinghe, K.
•
Moriarty, N. W.
...•
Afonine, P. V.
biorxiv
Mon Apr 07 2025
Surface Wetting Is a Key Determinant of α-Synuclein Condensate Maturation
-Synuclein self-assembles into amyloid fibrils during neurodegeneration. The protein can also self-assemble via liquid-liquid phase separation to form biomolecular condensates. The link between these processes is evident, as -synuclein condensates can mature into amyloids. However, the mechanisms driving this maturation remain largely unknown, particularly when incorporating pathological post-tran...
Thrush, R. J.
•
Vadukul, D. M.
•
Allerton, S. C.
•
Storch, M.
•
Aprile, F. A.
biorxiv
Sun Apr 06 2025
The Pseudomonas aeruginosa Membrane Histidine Kinase BqsS/CarS Directly Senses Environmental Ferrous Iron (Fe2+)
Canonical prokaryotic two-component signal transduction systems (TCSs) are widely utilized by bacteria to respond to their environment and are typically composed of a transmembrane sensor His kinase (HK) and a cytosolic DNA-binding response regulator (RR) that work together to respond to environmental stimuli. An important TCS that regulates the expression of genes involved in biofilm formation an...
Paredes, A.
•
Iheacho, C.
•
Chacon, K. N.
•
Smith, A. T.
biorxiv
Sun Apr 06 2025
Attenuative effect of Grifola frondosa (maitake mushroom) on severe DSS-induced colitis in vitamin D-deficient mice
Scope: Vitamin D deficiency, which has been a global health issue for decades, is involved in gut health and diseases. We examined the health benefits of Grifola frondosa (maitake mushroom), a potent dietary source of fungal vitamin D (D2), on DSS-induced colitis in vitamin D-deficient mice. Methods and results: C57BL/6J mice were fed a control diet, vitamin D3-deficient diet (DD), maitake-fortifi...
Nishikawa, M.
•
Miyagi, R.
•
Kugimiya, Y.
•
Chudan, S.
...•
Ikushiro, S.
biorxiv
Sat Apr 05 2025
Linoleic acid metabolite, 13-S-hydroxyoctadecadienoic acid, suppresses cancer cell growth by inhibiting mTOR
Mechanistic target of rapamycin (mTOR) is a key protein kinase that integrates various internal and external signals to control biological events including cell growth. Whereas substantial efforts were made to elucidate protein subunits interacting with mTOR, endogenous metabolite-mTOR interactions remain largely unknown. Using affinity protein purification and mass spectrometry, we identified dir...
Park, S. J.
•
Kwon, H.
•
Yang, A. Y.
•
Lee, B.
...•
Kim, S.
biorxiv
Sat Apr 05 2025
Comparison of Urinary Proteome Modifications Between Methamphetamine-Abstinent Rehabilitation Patients for Over Three Months and Healthy Individuals
Methamphetamine addiction is characterized by complex mechanisms and a high relapse rate, posing a serious public health threat. In this study, urinary proteome modifications of rehabilitation patients who had abstained from methamphetamine for over three months and those of healthy individuals were analyzed and compared. A total of 984 differentially modified peptides were identified, 362 of whic...
Chen, Y.
•
Shen, Z.
•
Liang, J.
•
Deng, Y.
•
Gao, Y.
biorxiv
Sat Apr 05 2025
Human endogenous retrovirus K (HERV-K) envelope structures in pre- and post-fusion by Cryo-EM
The HERV-K envelope glycoprotein (Env) is aberrantly expressed in diseases including cancers and autoimmune disorders and is targeted by antibodies. The lack of structural information has hindered functional and immune recognition studies. We solved structures of the HERV-K Env in both pre- and post-fusion states with novel monoclonal antibodies using cryo-EM. The pre-fusion Env assembles as a tri...
Shek, J.
•
Sun, C.
•
Wilson, E. M.
•
Moadab, F.
...•
Ollmann Saphire, E.
biorxiv
Fri Apr 04 2025
Complex carbohydrates catabolism capacity of bladder microbiota inhabiting healthy and overactive female bladders
Overactive bladder syndrome (OAB) is a poorly understood symptom complex that affects 40% of females over the age of 40, with clinical features including urinary urgency and incontinence. In addition to inflammation, oxidative stress, nerve damage and reduced blood flow, alterations in the urinary microbiome (urobiome), specifically in bladder bacterial diversity, have been reported to be associat...
Gourdine, J.-P.
•
Pacentine, I. V.
•
Barstad, A.
•
Dahl, E. M.
...•
Karstens, L.