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April 7th, 2025
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Centre for Ecological and Evolutionary Synthesis, Department of Biosciences, University of Oslo
genomics
biorxiv

Comparison of whole-genome assemblies of European river lamprey (Lampetra fluviatilis) and brook lamprey (Lampetra planeri)

Torresen, O. K.Open in Google Scholar•Garmann-Aarhus, B.Open in Google Scholar•Hoff, S. N. K.Open in Google Scholar•Jentoft, S.Open in Google Scholar•Svensson, M.Open in Google Scholar•Schartum, E.Open in Google Scholar•Tooming-Klunderud, A.Open in Google Scholar•Skage, M.Open in Google Scholar•Krabberod, A.Open in Google Scholar•Vollestad, L. A.Open in Google Scholaret al.

We present haplotype-resolved whole-genome assemblies from two individuals of the sister species the European river lamprey (Lampetra fluviatilis) and the brook lamprey (Lampetra planeri). The genome assemblies for L. fluviatilis consists of pseudo-haplotype one, spanning 1073 megabases and 963 megabases for pseudo-haplotype two. For L. planeri, the genome assemblies span 1049 megabases and 960 megabases for pseudo-haplotypes one and two, respectively. The river lamprey assemblies have been scaffolded into 82 pseudochromsomes for both pseudo-haplotypes, with the same number for L. planeri. All four haplotype assemblies were annotated, identifying 21,479 and 16,973 genes in pseudo-haplotypes one and two for L. fluviatilis, and 24,961 and 21,668 genes in pseudo-haplotypes one and two for L. planeri. A comparison of the genomes of L. fluviatilis and L. planeri, alongside a separate chromosome level assembly of L. fluviatilis from the UK, indicates that they form a species complex, potentially representing distinct ecotypes. This is further supported by phylogenetic analyses of the three reference Lampetra genomes in addition to sea lamprey.

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