2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
April 14th, 2025
Version: 1
Institut Pasteur
microbiology
biorxiv

Vector Competence of Temperate and Tropical Mosquito Species for Babanki virus

Ban, M.Open in Google Scholar•Geolier, V.Open in Google Scholar•Ferquel, E.Open in Google Scholar•Faye, M.Open in Google Scholar•Diagne, M. M.Open in Google Scholar•Ndiaye, E. H.Open in Google Scholar•Fall, G.Open in Google Scholar•Diallo, M.Open in Google Scholar•Lavillette, D.Open in Google Scholar•Choumet, V.Open in Google Scholar

Babanki virus (BBKV), an alphavirus belonging to the Western Equine encephalitis complex, was isolated from Mansonia africana mosquitoes in 1969 but is not characterized yet. Transmitted to humans and animals through the bite of an infectious female arthropod, it is associated with febrile illness, rash, and arthritis similarly to Sindbis virus to which it is related. No specific treatment or vaccine is available. To investigate any risk of propagation, our study investigated the vector competence of several mosquito species towards BBKV. For this purpose, laboratory mosquito colonies from metropolitan France: Culex pipiens (Paris), Aedes albopictus (Nice) as well as tropical area: Aedes albopictus (Saint-Benoit, La Reunion) and Culex quinquefasciatus (Slab strain), Aedes aegypti (PAEA) were exposed to an artificial blood meal infected with BBKV molecular clone. Midguts, legs, salivary glands and saliva were collected from individual mosquitoes of different strains or species at various time points to assess the infection status, viral replication, and infectiousness. At 7 days post viral exposure, infection rates exceeded 70% in Aedes and 40% in Culex species. They remain above 50% except for Culex quinquefasciatus which decreases to 35% at 14 days post viral exposure. Using genome and protein detection, the dissemination rates are above 50% except for Aedes albopictus (Nice) at 7 days post viral exposure. Infection assays using VeroE6 cells indicated that BBKV replicative viral particles were found in the saliva of the different populations tested. Our data demonstrate that different mosquitoes\' species from both temperate and tropical areas are competent to transmit BBKV suggesting an emergence risk that could trigger an outbreak in humans.

Similar Papers

biorxiv
Tue Apr 15 2025
Phage Steering in the Presence of a Competing Bacterial Pathogen
The rise of antibiotic-resistant bacteria has necessitated the development of alternative therapeutic strategies such as bacteriophage therapy, where viruses infect bacteria, reducing bacterial burden. However, rapid bacterial resistance to phage treatment remains a critical challenge, potentially leading to failure. Phage Steering, which leverages the evolutionary dynamics between phage and bacte...
Czerwinski, S. M.
•
Gurney, J.
biorxiv
Tue Apr 15 2025
Sulfoquinovose is exclusively metabolized by the gut microbiota and degraded differently in mice and humans
Sulfoquinovose (SQ) is a green-diet-derived sulfonated glucose and a selective substrate for few human gut bacteria. Complete anaerobic SQ degradation via interspecies metabolite transfer to sulfonate-respiring bacteria produces hydrogen sulfide, which has dose- and context-dependent health effects. Here, we studied potential SQ degradation by the mammalian host and the impact of SQ supplementatio...
Krasenbrink, J.
•
Hanson, B. T.
•
Weiss, A. S.
•
Borusak, S.
...•
Loy, A.
biorxiv
Tue Apr 15 2025
Shallow-water chemosymbiotic clams are a globally significant and previously overlooked carbon sink
Chemosynthetic animal symbioses are common in marine ecosystems but remain overlooked as contributors to global carbon fixation. We show that the shallow-water thyasirid clam Thyasira tokunagai, dominant in Yellow Sea sediments, harbors sulfur-oxidizing Sedimenticola symbionts with remarkably consistent genomic contents and functionality across the region, showing active Calvin cycle gene expressi...
Li, M.
•
Li, Y.
•
Mao, S.
•
Zhang, Z.
...•
Sun, J.
biorxiv
Mon Apr 14 2025
Nanocluster-antibody-drug conjugates (NADC) as an intravesical precision theranostic agent for interstitial cystitis
Interstitial cystitis (IC) is a chronic inflammatory bladder disorder with recurring severe pain, lacking timely diagnostic and therapeutic options. Here, we propose a unitary theranostic nanocluster-antibody-drug conjugate (NADC) by covalently placing dihydroorotate dehydrogenase inhibitors (DHODHi) and ultrasmall gold quantum clusters (AuQCs) on a nerve growth factor (NGF) antagonistic antibody ...
Lin, Z.
•
Wang, W.
•
Liu, D.
•
Liu, Q.
...•
Yang, J.
biorxiv
Mon Apr 14 2025
Calprotectin protects Staphylococcus aureus in coculture with Pseudomonas aeruginosa by attenuating quorum sensing and decreasing the production of pseudomonal antimicrobials
Pseudomonas aeruginosa and Staphylococcus aureus cause debilitating polymicrobial infections in diverse patient populations. Studies of these bacterial pathogens in coculture have shown that environmental variables including Fe availability and the host-defense protein calprotectin (CP) impact coculture dynamics. To decipher how CP modulates interactions between P. aeruginosa and S. aureus, we emp...
Lee, W. H.
•
Oglesby, A. G.
•
Nolan, E. M.
biorxiv
Mon Apr 14 2025
Degradation of resistant α-1,4-glucan by vaginal Gardnerella species is associated with bacterial vaginosis
This study investigates the degradation of resistant -1,4-glucan by vaginal bacterial species, with a focus on Gardnerella spp., to elucidate its role in bacterial vaginosis (BV). The ability of Gardnerella vaginalis, Gardnerella swidsinskii, Gardnerella leopoldii, Gardnerella piotii, Lactobacillus iners, and Lactobacillus crispatus was assessed to metabolize an ungelatinized, labeled form of raw ...
Hertzberger, R.
•
Morselli, S.
•
Botschuijver, S.
•
Himschoot, L.
...•
Kort, R.
biorxiv
Mon Apr 14 2025
Identifying a novel mechanism of L-leucine uptake in Mycobacterium tuberculosis using a chemical genomic approach
Amino acid biosynthesis is vital for Mycobacterium tuberculosis (Mtb) proliferation and tuberculosis pathogenesis. However, it is not clear how amino acids are transported in Mtb, particularly the branched chain amino acids (BCAAs) that contribute to the production of the cell-wall lipid component precursors such as acetyl-CoA and propionyl-CoA. While performing the screening of an FDA-approved re...
Agarwal, N.
•
Gogoi, H.
•
Eeba, E.
•
Augustin, L.
...•
Dey, B.
biorxiv
Mon Apr 14 2025
The Mycobacterium tuberculosis long-chain fatty acid resistome reveals the universal stress protein TB15.3 as essential for infection
The human pathogen Mycobacterium tuberculosis (Mtb) thrives in lipid-rich microenvironments. Long-chain fatty acids (LCFA) are some of the most abundant lipids encountered by Mtb during infection. Mtb has evolved to utilize LCFA as a preferred carbon source, however, LCFA are also known to be potent antimicrobials. Mtb must therefore employ mechanisms to utilize LCFA as a carbon source while avoid...
Pinto, A. J.
•
Silva, M.
•
Santos, J. A.
•
VanderVen, B. C.
...•
Beites, T.
biorxiv
Mon Apr 14 2025
Pleiotropic role of PAX cyclolipopeptides in the Xenorhabdus bacterium mutualistically associated with entomopathogenic nematodes
Xenorhabdus is an entomopathogenic bacterium involved in a mutualistic relationship with Steinernema nematodes. Xenorhabdus produces a multitude of specialized metabolites by Non-Ribosomal Peptide Synthetases (NRPSs) pathways to mediate bacterial-nematode-insect interactions. PAX cyclolipopeptides are a family of NRP-type molecules whose ecological role remains poorly understood. In this study, th...
Claveyroles, N.
•
Lanois-Nouri, A.
•
El Fannassi, I.
•
Ogier, J.-c.
...•
Givaudan, A.
biorxiv
Mon Apr 14 2025
Activation of the Pseudomonas aeruginosa Glycerol Regulon Promotes Antibiotic Persistence and Modulates Virulence Phenotypes
Chronic infections with Pseudomonas aeruginosa are a major contributor of lung decline in persons with cystic fibrosis (pwCF). P. aeruginosa establishes life-long infections in the CF airway by utilizing various adaptation strategies to persist, including altering the expression of metabolic genes to acquire nutrients that are abundant in the CF airway. Glycerol, which is readily available in the ...
Evans, N. J.
•
Scoffield, J. A.