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April 17th, 2025
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CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences
plant biology
biorxiv

STM is required for fate establishment of productive shoot progenitor cells in Arabidopsis tissue culture

Zhai, N.Open in Google Scholar•Xie, D.Open in Google Scholar•Xu, L.Open in Google Scholar

De novo shoot regeneration via the two-step tissue culture method is widely used for vegetative reproduction of plants and for gene transformation and editing. A key step in tissue culture is the fate transition from shoot progenitor cells (SPCs) to productive SPCs (prSPCs), which are capable of differentiating into a shoot. In this study, we identified the class I KNOTTED1-like homeobox (KNOX) transcription factor gene SHOOT-MERISTEMLESS (STM) as the key controller of, and marker gene for, the fate transition from SPCs into prSPCs, indicating that STM might serve as a candidate of molecular tools to improve de novo shoot regeneration in the agricultural applications.

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