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April 20th, 2025
Version: 1
University of Amsterdam
neuroscience
biorxiv

A polymeric, PDMS-based large-scale skull replacement suitable for optical and mechanical access for long-term neuronal imaging, electrophysiology, and optogenetics

Takahashi, K.Open in Google Scholar•Huis in 't Veld, G. J.Open in Google Scholar•Benedetti, D.Open in Google Scholar•Wang, J.-Y.Open in Google Scholar•Pontes Quero, S.Open in Google Scholar•Yuste, R.Open in Google Scholar•Pennartz, C. M. A.Open in Google Scholar•Olcese, U.Open in Google Scholar

Many techniques to record and manipulate neuronal activity across large portions of the vertebrate brain, such as widefield and two-photon calcium imaging, electrophysiology, and optogenetics, are now available. However, few effective approaches enable both optical and mechanical access to the brain. In this work, we offer an in-depth guide for synthesizing, implanting, and using polydimethylsiloxane (PDMS) windows as skull replacements for chronic optical neuronal imaging. Furthermore, we provide instructions to perform viral injections and multi-site silicon probe implantation.

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