2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
April 23rd, 2025
Version: 3
Frankfurt Institute for Advanced Studies
molecular biology
biorxiv

How does Mycoplasma pneumoniae steal lipids from its host membranes?

Manger, S.Open in Google Scholar•Arghittu, S. M.Open in Google Scholar•Sprankel, L.Open in Google Scholar•Meier-Credo, J.Open in Google Scholar•Wieland, K.Open in Google Scholar•Bublak, D.Open in Google Scholar•Langer, J.Open in Google Scholar•Covino, R.Open in Google Scholar•Frangakis, A. S.Open in Google Scholar

Lipid acquisition and transport are fundamental processes in all organisms. Here, we investigate the lipid uptake and delivery mechanism of the minimal model organism Mycoplasma pneumoniae. We show that the essential protein P116 can transport lipids between liposomes independently and without ATP consumption. Our structural data and molecular dynamics simulations reveal the mechanism by which the N-terminal region of P116 perturbs the membrane, the lipid transfer route, and the regulation of membrane binding by the cargo mass within P116's large hydrophobic cavity. When adequately filled with cargo, P116 undergoes a rapid conformational change that modulates membrane binding. Taken together, our results show that Mycoplasma developed one integrated lipid uptake and delivery machinery that simplifies the complex multi-protein pathways used by higher developed organisms.

Similar Papers

biorxiv
Fri Apr 25 2025
A genome-guided atlas to the composition, activity, and β-bungarotoxin dimerization in many-banded krait venom inferred by functional venomics
Snakebite is a neglected tropical disease claiming ~140,000 lives every year. One of the most medically relevant snakes of Asia is the many-banded krait (Bungarus multicinctus). Approximately 8% of the global human population is at risk of being envenomated by this species, able to cause fatal neurotoxicity. Here, we present a proteogenomic and functional assessment of the B. multicinctus venom vi...
Uhrig, L.
•
Avella, I.
•
Schulte, L.
•
Hurka, S.
...•
Lueddecke, T.
biorxiv
Fri Apr 25 2025
Chromatin regulator HELLS mediates SSB repair and responses to DNA alkylation damage.
The SNF2 family chromatin remodeler HELLS has emerged as an important regulator of cell proliferation, genome stability, and several cancer pathways. Significant upregulation of HELLS has been reported in 33 human cancer types. While HELLS has been implicated in DNA damage response, its function in DNA repair is poorly understood. Here we report a new regulatory link between HELLS and single-stran...
Joseph, J. T.
•
Wright, C. M.
•
Peixoto, E.
•
Khan, A.
...•
Czaja, W.
biorxiv
Fri Apr 25 2025
Specific origin selection and excess functional MCM2-7 loading in ORC-deficient cells.
The six subunit Origin Recognition Complex (ORC) loads excess MCM2-7 on chromosomes to promote initiation of DNA replication and is believed to be important for origin specification. Mapping of origins in cancer cell lines engineered to delete three of the subunits, ORC1, ORC2 or ORC5 shows that specific origins are still used and are mostly at the same sites in the genome as in wild type cells. T...
Shibata, Y.
•
Peycheva, M.
•
Shibata, E.
•
Malzl, D.
...•
Dutta, A.
biorxiv
Thu Apr 24 2025
Hangover regulates gene expression by limiting NSL-mediated H4K16 acetylation
The RNA-binding protein hangover is essential for several stress responses in Drosophila melanogaster. Here, we discover a novel function of hangover in the regulation of gene expression. Hangover binds to more than 2.000 genes in the Drosophila genome and modulates transcription. We identify a diverse set of chromatin regulators as hangover interactors, including NSL, dMec, Sin3A, dREAM and Ino80...
Lenz, J.
•
Schmelzer, L.
•
Forne, I.
•
Nist, A.
...•
Brehm, A.
biorxiv
Thu Apr 24 2025
The co-receptors Orco and Ir8a are required for coordinated expression of chemosensory genes in the antennae of the yellow fever mosquito, Aedes aegypti.
Olfaction has been extensively studied in the yellow fever mosquito, Aedes aegypti. This species uses its sense of smell to find blood hosts and other resources, con-tributing to its impact as a vector for human pathogens. Two major families of pro-tein-coding genes, the odorant receptors (Ors) and the ionotropic receptors (Irs), provide the mosquito with sensitivities to distinct classes of volat...
Cooke, M. M.
•
Chembars, M. S.
•
Pitts, R. J.
biorxiv
Thu Apr 24 2025
Deciphering ESR1-driven transcription in human endometrial stromal cells via transcriptome, cistrome, and integration with chromatin landscape
Objective: To investigate ESR1 and estrogen-driven transcription in human endometrial stromal cells. Design: Telomerase-immortalized human endometrial stromal cells were engineered to activate ESR1 using the CRISPR activation system and treated with vehicle or E2. Primary endometrial stromal cells were treated with vehicle or decidualization cocktail. Subjects: Biopsies from two healthy, reproduct...
Montague Redecke, S. G.
•
Bell-Hensley, A.
•
Li, S.
•
Jain, A.
...•
DeMayo, F. J.
biorxiv
Thu Apr 24 2025
A pre-loaded and assembled human Ago2-miRISC orchestrates Shigella-induced vacuolar rupture through the targeting of RhoGDIα
The enteroinvasive bacterium Shigella flexneri causes bacillary dysentery. It invades intestinal epithelial cells and forms a bacterium-containing vacuole, which it rapidly ruptures to reach the cytosol. Here, we found that human Argonaute 2 (Ago2), the central component of miRNA-induced silencing complex (miRISC), positively regulates early steps of Shigella infection, including vacuolar rupture....
Navarro, L.
•
Schiavolin, L.
•
Filopon, D.
•
Bonnet, M.
...•
TRAN VAN NHIEU, G.
biorxiv
Thu Apr 24 2025
A genome-guided atlas to the composition, activity, and β-bungarotoxin dimerization in many-banded krait venom inferred by functional venomics
Snakebite is a neglected tropical disease claiming ~140,000 lives every year. One of the most medically relevant snakes of Asia is the many-banded krait (Bungarus multicinctus). Approximately 8% of the global human population is at risk of being envenomated by this species, able to cause fatal neurotoxicity. Here, we present a proteogenomic and functional assessment of the B. multicinctus venom vi...
Uhrig, L.
•
Avella, I.
•
Schulte, L.
•
Hurka, S.
...•
Lueddecke, T.
biorxiv
Wed Apr 23 2025
Optimised genome editing for precise DNA insertion and substitution using Prime Editors in zebrafish
CRISPR/Cas9-mediated genome editing has rapidly become a popular tool for studying gene functions and generating genetically modified organisms. However, using this system, stochastic integration of random insertions and deletions restricts precise genome manipulation. Advanced CRISPR/Cas9 technologies using Prime Editors (PEs), Cas9 proteins fused with reverse transcriptase, enable programmed int...
Ono, Y.
•
Peterka, M.
•
Love, M.
•
Bhandari, A.
...•
Scholpp, S.