2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
May 7th, 2025
Version: 2
CEITEC MU - Central European Institute of Technology, Masaryk University, Brno, Czech Republic
plant biology
biorxiv

MONOPTEROS isoform MP11ir role during somatic embryogenesis in Arabidopsis thaliana

Wojcikowska, B.Open in Google Scholar•Belaidi, S.Open in Google Scholar•Mironova, V.Open in Google Scholar•Citerne, S.Open in Google Scholar•Robert, H. S.Open in Google Scholar

Auxin is crucial for plant morphogenesis, including in vivo and in vitro embryo development. Exogenous auxin application is necessary for inducing embryogenic responses in in vitro cultured explants of Arabidopsis and other plants. Thus, components of auxin transport, signaling, and metabolism are key to somatic embryogenesis. AUXIN RESPONSE FACTOR (ARF) transcription factors bind to auxin response elements to control auxin-responsive gene expression and are vital in somatic embryo regeneration. ARFs are often repressed by AUXIN/INDOLE-3-ACETIC ACIDs (Aux/IAAs). MONOPTEROS (MP)/ARF5 is especially important in the embryogenic transition, being highly expressed during somatic embryogenesis; its mutant cannot develop somatic embryos. The MP11ir transcript, an alternatively spliced variant of MP, produces a truncated protein missing the Phox and Bem1p (PB1) domain, crucial for ARF-Aux/IAA dimerization. This renders the MP11ir isoform insensitive to Aux/IAA repression, suggesting an auxin-independent regulation. High levels of MP11ir transcript are observed during auxin- and trichostatin A-dependent induction of somatic embryogenesis. Both MP and MP11ir are essential for embryo regeneration in the mpS319 mutant. However, overexpression of a truncated MP protein ({Delta}ARF5) lacking the PB1 domain inhibits somatic embryogenesis, resulting in callus formation instead of somatic embryos. Overexpression of {Delta}ARF5, lack of MP protein (mp mutant), and interference with MP action by the auxin-resistant BODENLOS (BDL) protein affect the expression of the genes TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS 1 (TAA1), TAA1-RELATED 1 (TAR1), YUCCA3 (YUC3), YUC5 and YUC8, which are involved in auxin biosynthesis. The partial complementation of mpS319 by the expression of MP11ir may be due to the restoration of the expression of TAA1, TAR1, YUC3, YUC5, and YUC8 to the levels observed in the wild-type genotype Col-0. The presence of MP protein alone or in combination with MP11ir in mpS319 restored the expression of all analyzed genes compared to Col-0. Our results suggest that the analyzed auxin-related genes could be potential targets of MP11ir and/or MP. Consequently, {Delta}ARF5 overexpression alters auxin homeostasis and endogenous auxin levels, hindering embryogenic transition.

Similar Papers

biorxiv
Thu May 08 2025
Floral Stage Optimization and Immune Evasion Enhance Agrobacterium-Mediated Genome Editing in Arabidopsis
Agrobacterium-mediated transformation via floral inoculation (AMT-FI) enables genetic engineering without tissue culture. It is widely used in the model plant Arabidopsis thaliana, yet its efficiency and broader applicability remain limited. Here, we identify key floral stages and develop novel strategies to enhance AMT-FI-based genome editing. Using a dual-reporter system (RUBY and hygromycin res...
Liu, M.-S.
•
Huang, T.-K.
•
Wang, Y.-C.
•
Wang, S.-C.
...•
Lai, E.-M.
biorxiv
Thu May 08 2025
G3BP1 Phosphorylation Regulates Plant Immunity in Arabidopsis
Mitogen-activated protein kinase (MAPK) cascades play critical roles in plant immunity by phosphorylating downstream effectors that regulate stress responses. While MAPK-mediated transcriptional regulation has been well examined, the involvement of MAPKs in post-transcriptional and post-translational regulation is still poorly understood. In this study, we identify the RNA binding-protein AtG3BP1 ...
Hirt, H.
•
Abdulhakim, F.
•
Abdulfaraj, A.
•
Rayapuram, N.
biorxiv
Thu May 08 2025
The Arabidopsis WAVE/SCAR Protein BRICK1 Associates with Cell Edges and Plasmodesmata
Plasmodesmata (PD) are specialized structures in plant cell walls that mediate intercellular communication by regulating the trafficking of molecules between adjacent cells. The actin cytoskeleton plays a pivotal role in controlling PD permeability, but the molecular mechanisms underlying this regulation remain unclear. Here, we report that BRK1, a component of the WAVE/SCAR complex involved in Ar...
Ambrose, C.
•
Chi, Z.
biorxiv
Tue May 06 2025
Liquid-phase determination of Arabidopsis respiration and photosynthesis using Clark-type O2 electrodes
Photosynthesis and respiration are fundamental metabolic processes in plants, tightly connected through shared substrates, energy dynamics, and redox balance. Arabidopsis is the key genetic model for plants but monitoring these sorts of physiological processes presents significant challenges using traditional gas-exchange or fluorescence-based techniques due to the small size of intact Arabidopsis...
Sena, F.
•
Couture, C.
•
Berais-Rubio, A.
•
Millar, A. H.
•
Signorelli, S.
biorxiv
Tue May 06 2025
Phylogenetic Analysis and Machine Learning Identify Signatures of Selection and Predict Deleterious Mutations in Common Bean
Mutations are continuous source of new alleles and genetic diversity in populations. Domestication and selection influence the accumulation of alleles occurring across a range of deleteriousness. Evidence suggests that mildly deleterious mutations (DelMut) can be purged out of breeding populations, increasing favorable allele accumulation. We used phylogeny-based analyses among 36 legume genomes t...
Cordoba-Novoa, H. A.
•
Buckler, E. S.
•
Romay, C.
•
Berthel, A.
...•
Hoyos Villegas, V.
biorxiv
Tue May 06 2025
Open RGB Imaging Workflow for Morphological and Morphometric Analysis of Fruits using AI: A Case Study on Almonds.
High-throughput phenotyping is addressing the current bottleneck in phenotyping within breeding programs. Imaging tools are becoming the primary resource for improving the efficiency of phenotyping processes and providing large datasets for genomic selection approaches. The advent of AI brings new advantages by enhancing phenotyping methods using imaging, making them more accessible to breeding pr...
Mas-Gomez, J.
•
Rubio, M.
•
Dicenta, F.
•
Martinez-Garcia, P. J.
biorxiv
Tue May 06 2025
Mechanisms and Plasticity in Leaves and Leaflets in a Creeping Legume, Mimosa pudica
Mimosa pudica (Fabaceae) is a creeping plant known for its rapid thigmonastic movement upon touch, facilitated by specialized joint-like thickenings called pulvini. This study examines the activation behavior of the primary pulvinus (P1) in response to repeated touch stimuli, providing evidence for a mechanical exhaustion mechanism underlying the response. Experiments were conducted on M. pudica i...
Kellogg, M. T.
biorxiv
Tue May 06 2025
A trio-binning approach for Cannabis genome de novo assembly reveals extensive structural variation, and defines paralog cohorts with very good resolution
With the advent of long read DNA sequencing technologies, assembling eukaryotic genomes has become routine; however, properly phasing the maternal and paternal contributions remains technically challenging. Here, we use the trio-binning approach to separate Oxford Nanopore reads derived from a Cannabis F1 wide cross, made between the Colombian landrace Punto Rojo and the Colorado CBD clone Cherry ...
Pike, B.
•
Kozik, A.
•
Teran, W.
biorxiv
Tue May 06 2025
A Circadian Light Regulator Controls a Core CAM Gene in the Ice Plant's C3-to-CAM Transition
Crassulacean acid metabolism (CAM) enhances drought tolerance by shifting carbon fixation to the night, improving water-use efficiency compared to C3 and C4 photosynthesis. However, the molecular regulators of CAM induction remain poorly understood. Here, we generate the first single-nucleus transcriptome atlas of a CAM species, Mesembryanthemum crystallinum, to resolve transcriptional dynamics at...
Perron, N.
•
Le, T.
•
Dervinis, C.
•
Pereira, W. J.
...•
Kirst, M.