The domesticated species currently available in the market have been developed through the breeding of wild relatives. Breeding strategies using wild relatives with high genetic diversity are attracting attention as an important approach for ad-dressing climate change and ensuring sustainable food supply. However, studies examining gene expression variation in multiple wild and domesticated species are few. Therefore, we aimed to investigate the changes in gene expression associated with domestication. We performed a meta-analysis of public gene expression data of domesticated species of rice, tomato, and soybean and their presumed ancestral species. In wild relatives, the expression of genes involved in osmotic, drought, and wound stress tolerance was upregulated. In domesticated species, upregulated expression was observed in genes related to auxin and those involved in the efflux of heavy metals and harmful substances. These findings provide insights into how domestication influences changes in crop traits. Thus, our findings may contribute to rapid breeding and development of new varieties capable of growing in harsh natural environments. Hence, a new cultivation method called \"de novo domestication\" has been proposed, which combines the genetic diversity of currently unused wild relatives and wild relatives with genome editing technologies that enable rapid breeding.