2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
June 29th, 2025
Version: 1
AO Research Institute Davos, Switzerland
bioengineering
biorxiv

Bioconvergence of sound-guided and supramolecular assembly strategies to create peptide-protein composite hydrogels with predictable shape-to-function features

Ligorio, C.Open in Google Scholar•Cianciosi, A. R.Open in Google Scholar•Tognato, R.Open in Google Scholar•Natta, M.Open in Google Scholar•Parolini, R.Open in Google Scholar•Ardicli, S.Open in Google Scholar•Babayev, H.Open in Google Scholar•Sapjanskaite, I.Open in Google Scholar•Kilgour, S.Open in Google Scholar•Homer, R.Open in Google Scholaret al.

Purely protein-based hydrogels are widely used in tissue engineering for their biomimicry and biocompatibility, yet remain challenging to tailor with precision and predictability at biological and mechanical levels. To overcome this, synthetic self-assembling peptide amphiphiles (PAs) offer opportunities for supramolecular customization, both as single-phase materials and co-assembled with proteins to create hybrid nanocomposites with emerging functionalities. Similarly, contactless, sound-guided bioassembly techniques using liquid-phase hydrogel precursors are emerging as strategic tools for obtaining structured and functional hydrogels. Leveraging these advances, here a fast, contactless, \"one-pot\" bioassembly strategy merging supramolecular PA self-assembly with sound-guided patterning to fabricate hybrid peptide-protein hydrogels with programmable shape-to-function features is presented. Using fibrin as proof-of-concept, material performance is biologically enhanced by incorporating growth factor-binding PAs, while inorganic microparticles are embedded and spatially organized via acoustic fields to tune mechanical properties. This strategy allows predictable tuning of composite stiffness and architecture by adjusting sound wave frequency, with acoustic fields guiding material organization from micro-to-macroscale. Composite hydrogels result highly permissive to cell infiltration in vitro and versatile platform to tune immune cell-material interactions. This modular biofabrication platform integrating supramolecular and sound-guided processes can be generalized to other building blocks opening unique opportunities for scalable, tunable, and hierarchically-organized biomaterials.

Similar Papers

biorxiv
Mon Jun 30 2025
Automated muscle path calibration with gradient-specified optimization based on moment arm
Objective: Muscle path modeling is more than just routing a cable that visually represents the muscle, but rather it defines how moment arms vary with different joint configurations. The muscle moment arm is the factor that translates muscle force into joint moment, and this property has an impact on the accuracy of musculoskeletal simulations. However, it is not easy to calibrate muscle paths bas...
Chen, Z.
•
Hu, T.
•
Haddadin, S.
•
Franklin, D.
biorxiv
Mon Jun 30 2025
Light-orchestrated multi-step solid-phase picodroplet reactors
Picoliter droplet reactors, enabled by droplet microfluidics, are revolutionary tools for biochemical reactions with high efficiency, precision, and minimal reagent use. They excel in single-step reactions and reagent addition through droplet merging. Integrating reagent exchange and washing capabilities directly into these platforms may enable complex multi-step processes, such as de novo oligonu...
Wu, M.
•
Zaman, M. A.
•
Jensen, M. A.
•
Padhy, P.
...•
Hesselink, L.
biorxiv
Mon Jun 30 2025
Recapitulating physiologically relevant oxygen levels and extracellular matrix remodeling in patient-derived tumor-immune tunable models reveal targeting opportunities for immunologically cold high-grade serous tumors
High-grade serous tumors are immunologically cold, characterized by limited immune cell infiltration and reduced clinical outcome, primarily due to hypoxia and extensive extracellular matrix remodeling that disrupt tumor-stromal-immune interactions. However, current experimental models fail to fully capture oxygen and matrix microenvironmental features, limiting progress in understanding tumor-imm...
Plesselova, S.
•
Axemaker, H.
•
Calar, K.
•
Isaiah, O.
...•
de la Puente, P.
biorxiv
Mon Jun 30 2025
Alginate hydrogels loaded with pyruvate dehydrogenase enzyme: a novel wound dressing to disassemble Pseudomonas aeruginosa biofilm
Topical wounds require effective dressings to manage wound exudate, maintain moisture, and avoid infection. Alginate hydrogels are widely used clinically due to their biocompatibility, high absorbency, and ability to maintain a moist wound environment during healing; however, alginate can also promote bacterial biofilm formation and prolong healing. Depleting pyruvate using the enzyme pyruvate deh...
Sedighi, O.
•
Roode, N.
•
Johnsen, T.
•
Moses, M.
...•
Doiron, A.
biorxiv
Mon Jun 30 2025
Engineering Versatile Two-Dimensional Nanobody-Origami Architectures for Enhanced Antiviral Activity
Considering the serious global health burden posed by pathogenic viruses, the development of effective antiviral molecules and therapeutic strategies is critical for improving human health. Designing and synthesizing a versatile and biocompatible molecular platform offering neutralization of both coronaviruses and retroviruses, two virus families that are greatly problematic to the human populatio...
Song, T.
•
Achi, J. G.
•
Anirudhan, V.
•
Le, L. T. P.
...•
Wang, X.
biorxiv
Mon Jun 30 2025
Engineered and decellularized human cartilage graft exhibits intrinsic immuno-evasive properties and full skeletal repair capacity
Tissue engineering strategies predominantly consist of the autologous generation of living substitutes capable of restoring damaged body parts. Persisting challenges with patient-specific approaches include inconsistent performance, high costs and delayed graft availability. Towards developing a one-for-all solution, a more attractive paradigm lies in the exploitation of dedicated cell lines for t...
Garcia Garcia, A.
•
Prithiviraj, S.
•
Raina, D. B.
•
Schmidt, T.
...•
Bourgine, P. E.
biorxiv
Sun Jun 29 2025
Development of a wireless bioelectronic actuator for wound healing in a porcine model
Wireless bioelectronic actuators have been developed to deliver targeted treatments over multiple days while continuously monitoring delivery, thereby improving wound healing. Specifically, these devices can deliver charged biomolecules such as fluoxetine cations (Flx+) and electric field (EF) in freely moving pigs. Treatments can be controlled and monitored in real time via WiFi, with options for...
Baniya, P.
•
Tebyani, M.
•
Hee, W. S.
•
Li, H.
...•
Teodorescu, M.
biorxiv
Sat Jun 28 2025
In vivo Calcium Imaging in the Near-Infrared II Window
Non-invasive deep-tissue calcium imaging of live mammals with high sensitivity and resolution is challenging owing to light scattering experienced by traditional calcium ion (Ca2+) indicators with excitation and emission wavelengths within 400-750 nm. Here, we report near-infrared II (NIR-II) calcium imaging beyond 1000 nm by exploring a natural protein derived from a bacterium (Thermochromatium t...
XU, D.
•
Wu, Z.
•
Li, W. J.
•
Yue, X.
...•
Wang, F.
biorxiv
Sat Jun 28 2025
Rictor CRISPR Gene Editing by Lipid Nanoparticle Delivery Stimulates Anti-Tumor Immunity in Breast Cancer Liver Metastasis Model
Targeting myeloid cells to remodel the immunosuppressive tumor microenvironment (TME) represents a promising strategy for cancer therapy. Here, we developed ionizable lipid nanoparticles (LNPs) engineered to deliver the CRISPR-Cas12a ribonuclease complex targeting Rictor, a critical component of mTORC2 and a key immunosuppression factor, for in vivo reprogramming of myeloid cells. Systemic deliver...
Ali, Y.
•
Galbraith, T.
•
Abdelfattah, N. H.
•
Ziemys, A. H.
...•
Leonard, F.