2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
July 1st, 2025
Version: 1
Royal Holloway, University of London
plant biology
biorxiv

An ARF2-GRF5 module, operating early and upstream of GLK factors, reveals genetic hierarchy during chloroplast biogenesis

Mishra, P.Open in Google Scholar•Hibberd, J. M.Open in Google Scholar•Lopez-Juez, E.Open in Google Scholar

During photosynthetic development, GOLDEN2-LIKE (GLK) factors drive the expression of photosynthesis-associated nuclear (PhAN) genes. Simultaneous loss of GLK1 and GLK2 in Arabidopsis leads to pale-green plants with small, incompletely developed chloroplasts, revealing involvement of additional components. Following mutagenesis of a glk1-1 knock-down, glk2-1 knock-out (KO) mutant we identified suppressor of glks (sgl) 2, sgl2. The sgl2 glk1 glk2 triple mutant showed increases in chlorophyll, PhAN gene transcripts and thylakoid stacking in mesophyll cell chloroplasts, and enhanced cellular content of chloroplasts in mesophyll and bundle-sheath cells. The suppressor mutation delayed flowering but greening phenotypes preceded flowering. sgl2 was caused by loss of function of AUXIN RESPONSE FACTOR2, ARF2. arf2sgl2 boosted chloroplast content. It dramatically suppressed the developmental and chloroplast delay of the phytochrome-deficient long hypocotyl 1 (hy1) mutant. We observed in glk1-1 glk2-1 low but detectable expression of GLK1, which was elevated in arf2sgl2 glk1-1 glk2-1, and saw reduced suppression by arf2sgl2 of a glk1-2 glk2-2 mutant carrying KO alleles, revealing partial epistasis. Overexpression of Growth Regulating Factor 5 (GRF5), upregulated in arf2sgl2, rescued chlorophyll levels in glk1-1 glk2-1, consistently with ARF2 and GRF5 as a transcription module. In developing cereal leaves presumed orthologs of ARF2 and GRF5 are expressed at early stages of plastid proliferation and assembly, preceding GLK expression during greening stage. ARF2 is therefore a negative switch modulating GRF5 early in chloroplast development in photosynthetic cells, upstream of the GLKs. Our results reveal a hierarchy of transcription factor action and feed-forward regulation in chloroplast biogenesis during leaf development.

Similar Papers

biorxiv
Tue Jul 01 2025
A TNL receptor mediates microbiome feedbacks in Arabidopsis
Plant performance depends on the soil microbiome. While microbiome feedbacks are well documented, the mechanisms by which plants perceive and mediate these feedbacks remain unclear. We established a framework using two distinct microbiomes in the same soil, where one led to enhanced growth of the Arabidopsis thaliana accession Col-0. Screening 410 accessions revealed substantial variation in growt...
Janse van Rensburg, H.
•
Schandry, N.
•
Waelchli, J.
•
Stengele, K.
...•
Schlaeppi, K.
biorxiv
Tue Jul 01 2025
Pollinate to Suppress: Sterile Pollen and Certation Effects in Palmer amaranth (Amaranthus palmeri)
The sterile pollen technique, which involves applying X-ray irradiated pollen to female plants, has shown promising results in reducing seed production in dioecious Palmer amaranth (Amaranthus palmeri S. Wats.). However, field-scale implementation of this method requires a carrier for pollen delivery, as applying pure pollen on a large scale is not practical. Additionally, variability in flowering...
Wu, W.
•
Mesgaran, M. B.
biorxiv
Tue Jul 01 2025
Cell-type-specific execution of effector-triggered immunity
Effector-triggered immunity (ETI) is a central component of host defense, but whether all cell types execute ETI similarly remains unknown. We combined chemically imposed immune activation with single-cell transcriptomics to profile ETI responses across all leaf cell types in Arabidopsis. Despite uniform ETI perception, we find striking divergence between transcriptional outputs: a core set of def...
Chhillar, H.
•
Jo, L.
•
Redkar, A.
•
Kajala, K.
...•
Ding, P.
biorxiv
Tue Jul 01 2025
Mycotoxin-driven proteome remodeling reveals limited activation of Triticum aestivum responses to emerging chemotypes integrated with fungal modulation of ergosterols
Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is a globally important wheat disease reducing yield and grain quality. The pathogen produces mycotoxins deoxynivalenol (DON), 3-acetyl DON (3ADON), 15-acetyl DON (15ADON), and nivalenol (NIV), which threaten food and feed safety. During the past 15 years, surveillance has identified nove trichothecenes 3ANX and NX, which show incr...
Ramezanpour, S.
•
Alijanimamaghani, N.
•
McAlister, J. A.
•
Hooker, D.
•
Geddes-McAlister, J.
biorxiv
Tue Jul 01 2025
Transcriptome Reprogramming Associated with Autoimmunity Reveals Key Points of the Growth-Defence Trade-off in Arabidopsis thaliana
Growth and defence are inversely correlated processes requiring balance for optimal fitness. While each is well studied, their regulatory trade-offs are less understood. Using DANGEROUS MIX (DM) autoimmune plants with stunted growth in Arabidopsis thaliana, we investigated the balancing act. Transcriptome analysis of three DM cases and pathogen-treated seedlings identified two major modules repres...
HU, D.
•
Wang, J.
•
Lee, R. R. Q.
•
Su, Z.
...•
Chae, E.
biorxiv
Tue Jul 01 2025
A canonical chloroplast unfolded protein response triggered by misfolded polypeptides
Photosynthetic efficiency and plant viability rely on chloroplast protein homeostasis. While unfolded protein responses (UPRs) in the endoplasmic reticulum and mitochondria have been extensively characterized, the chloroplast UPR (cpUPR) remains less defined, partly due to the off-target effects of traditional stress-inducing methods. In this study, we provide direct evidence for the existence of ...
Cantoia, A.
•
Bertero, F.
•
Berrocal, R.
•
Ceccarelli, E. A.
...•
Rosano, G. L.
biorxiv
Tue Jul 01 2025
A Transcriptomic Atlas of Macauba Palm Reveals Organ-Specific Gene Expression and Stress-Related Pathways
The macauba palm (Acrocomia aculeata) is an emerging oilseed species with promising applications in biodiesel production, as well as in food and cosmetic industries. Native to the Neotropical, it is incipiently domesticated and distributed across diverse environments and edaphoclimatic conditions. However, genomic studies of macauba are limited due to the scarcity of publicly available sequence da...
de Carvalho, L. M.
•
Bazzo, B. R.
•
Shanley, C. C.
•
Colombo, C. A.
...•
Carazzolle, M. F.
biorxiv
Tue Jul 01 2025
Unlocking Genetic Diversity in Colombian Cassava Landraces for Accelerated Breeding
Cassava (Manihot esculenta Crantz) is a staple food for hundreds of millions across the global south. In this study, we investigated genomic diversity among over 1000 cassava genotypes, with a particular focus on the addition of 387 newly sequenced landrace varieties originating from diverse climates across Colombia. As cassava was domesticated in or near the Amazon basin, these landraces represen...
Zhao, K.
•
Long, E.
•
Sanchez, F.
•
Chavarriaga, P.
•
Monroe, J. G.
biorxiv
Tue Jul 01 2025
Ligand-induced ubiquitination regulates endocytosis and homeostasis of the ERECTA receptor kinase for stomatal development
Stomata, valves on the plant epidermis, control gas and water vapor exchange. The patterning and spacing of stomata are primarily regulated by the ERECTA leucine-rich repeat receptor kinase (LRR-RK). Ubiquitination is a crucial mechanism to regulate the homeostasis of RKs by impacting their protein stability and localization. It has been shown that plant U-box ubiquitin E3 ligases, PUB30 and PUB31...
Chen, L.
•
Vu, M. H.
•
Cochran, A. M.
•
Ying, C. F.
•
Torii, K. U.