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July 2nd, 2025
Version: 3
The Salk Institute, University of Miami
plant biology
biorxiv

Medicago truncatula CORYNE modulates inflorescence meristem branching, nutrient signaling, and arbuscular mycorrhizal symbiosis

Orosz, J.Open in Google Scholar•Lin, E. X.Open in Google Scholar•Torres Ascurra, Y. C.Open in Google Scholar•Kappes, M.Open in Google Scholar•Lindsay, P. L.Open in Google Scholar•Bashyal, S.Open in Google Scholar•Everett, H.Open in Google Scholar•Gautam, C. K.Open in Google Scholar•Jackson, D.Open in Google Scholar•Mueller, L. M.Open in Google Scholar

The CLAVATA signaling pathway regulates plant development and plant-environment interactions. CLAVATA signaling consists of mobile, cell-type or environment-specific CLAVATA3/ESR-related (CLE) peptides, which are perceived by a receptor complex consisting of leucine-rich repeat receptor-like kinases such as CLAVATA1 and receptor-like proteins such as CLAVATA2, which often functions with the pseudokinase CORYNE (CRN). CLAVATA signaling has been extensively studied in various plant species for its role in meristem maintenance and in legumes for modulating root interactions with nitrogen-fixing rhizobia. Some signaling proteins involved in development and nodulation, including CLAVATA1, also regulate plant interactions with mutualistic arbuscular mycorrhizal (AM) fungi. However, our knowledge on AM symbiosis regulation by CLAVATA signaling remains limited and only a handful of genetic regulators have been identified. Here we report that Medicago truncatula CRN controls inflorescence meristem branching and negatively regulates root interactions with AM fungi. MtCRN functions partially independently of the AM autoregulation signal MtCLE53. Transcriptomic data revealed that crn roots display signs of perturbed nutrient, symbiosis, and stress signaling, suggesting that MtCRN plays various roles in plant development and interactions with the environment.

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