2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
July 3rd, 2025
Version: 1
Universitat Politecnica de Catalunya-BarcelonaTech
biophysics
biorxiv

Multiscale wrinkling dynamics in epithelial shells

Chahare, N.Open in Google Scholar•Ouzeri, A.Open in Google Scholar•Wilson, T.Open in Google Scholar•Bal, P. K.Open in Google Scholar•Golde, T.Open in Google Scholar•Vilanova, G.Open in Google Scholar•Pujol-Vives, P.Open in Google Scholar•Roca-Cusachs, P.Open in Google Scholar•Trepat, X.Open in Google Scholar•Arroyo, M.Open in Google Scholar

Thin shells buckle and wrinkle when compressed. While this behavior is generally detrimental in engineering, it has been widely implicated in epithelial morphogenesis and patterning during development. Yet the rules governing buckling of active viscoelastic shells like epithelia remain unclear. Here we delineate those rules by combining an experimental system that allows us to sculpt epithelial shells and subject them to controlled deflation with a 3D computational model linking cytoskeletal dynamics to tissue mechanics. Experiments and simulations across several orders of magnitude in time and space reveal that buckling emerges for fast deflation relative to the cortex\'s relaxation time, and is suppressed by high contractility. We show, further, that the tissue develops wrinkle patterns with different degrees of symmetry breaking that depend on its size and viscous confinement. Strikingly, we find that epithelial buckling is a multiscale phenomenon involving long-lived supracellular folds but also short-lived subcellular wrinkles in the actin cortex. Finally, by forming epithelial shells with anisotropic curvature we rationally direct buckling into predictable wrinkle patterns. Our study shows that epithelial tissues can be understood as hierarchical materials with mechanical instabilities that can be harnessed to engineer epithelial morphogenesis.

Similar Papers

biorxiv
Thu Jul 03 2025
Phototactic Decision-Making by Micro-Algae
We study how simple eukaryotic organisms make decisions in response to competing stimuli in the context of phototaxis by the unicellular alga Chlamydomonas reinhardtii. While negatively phototactic cells swim directly away from a collimated light beam, when presented with two beams of adjustable intersection angle and intensities, we find that cells swim in a direction given by an intensity-weight...
Raikwar, S.
•
Al-Kassem, A.
•
Gov, N. S.
•
Pesci, A.
...•
Goldstein, R. E.
biorxiv
Thu Jul 03 2025
Multiparametric Correlative Topographical and Volumetric Fluorescence Microscopy
Live-cell imaging of cell surface topography and intracellular architecture is essential for understanding cellular function. However, conventional approaches often involve trade-offs between resolution, invasiveness, and volumetric coverage. Here, we present an integrated Scanning Ion Conductance Microscope and single-objective Oblique Plane Microscope (SICM-OPM) system that enables simultaneous ...
Hong, W.
•
Zhang, Z.
•
Li, A.
•
Sun, T.
...•
Shevchuk, A.
biorxiv
Thu Jul 03 2025
Modelling mechanochemical coupling in optogenetically activated cell layers
In adherent cells, actomyosin contractility is regulated mainly by the RhoA signaling pathway, which can be controlled by optogenetics. To model the mechanochemical coupling in such systems, we introduce a finite element framework based on the discontinuous Galerkin method, which allows us to treat cell doublets, chains of cells and monolayers within the same conceptual framework. While the adhere...
Wörthmüller, D.
•
Ziebert, F.
•
Schwarz, U. S.
biorxiv
Thu Jul 03 2025
Why are so many fusogens rod-shaped?
Molecular fusogens catalyze membrane fusion for many basic biological processes. In eukaryotic cells, SNARE proteins drive membrane fusion for trafficking and for exocytic release in many contexts, from neurotransmission to enzymatic digestion, while other fusogens mediate cell-cell fusion for organ formation, placental development and gamete fusion. Enveloped viruses use glycoprotein fusogens for...
Butu, I. C.
•
Zeng, J.
•
An, D.
•
McDargh, Z. A.
...•
O'Shaughnessy, B.
biorxiv
Wed Jul 02 2025
Structural Basis for Efficient Fo Motor Rotation Revealed by MCMD simulation and Structural Analysis
Fo domain of ATP synthase functions as a rotary molecular motor, coupling proton translocation with the rotation of the c-ring rotor. This process involves proton uptake at the entry half channel, rotor rotation, and proton release to the exit half channel. While the overall coupling mechanism is established, the design principle for efficient rotation remains unclear. Here, we employed hybrid mol...
Kubo, S.
•
Noji, H.
biorxiv
Wed Jul 02 2025
Frequency-dependent cellular microrheology with pyramidal atomic force microscopy probes
Atomic force microscopy (AFM) is widely used to measure the elastic properties of living cells, typically by extracting the Young\'s (elastic) modulus from force-indentation curves acquired at a fixed loading rate. However, it is increasingly clear that cells relax applied stresses over time and exhibit significant frequency-dependent loss properties, a phenomenon which figures centrally in many b...
Ding, E.
•
Kumar, S.
biorxiv
Wed Jul 02 2025
Conformational flexibility of tubulin dimers regulates the transitions of microtubule dynamic instability
Microtubules are highly conserved polymers of {beta}-tubulin dimers that undergo dynamic instability. While dynamic instability is conserved across eukaryotes, many of its associated conformational changes, like lattice compaction and twist, are not. Tubulin dimers sample multiple conformations in solution and undergo conformational changes during polymerization; the rate and extent of these chang...
Shred, M.
•
Vangos, N. E.
•
Bayne, A. N.
•
Tetlalmatzi, S. C.
...•
Brouhard, G.
biorxiv
Tue Jul 01 2025
All You Need Is Water: Converging Ligand Binding Simulations with Hydration Collective Variables
Selecting appropriate collective variables (CVs) is a crucial bottleneck in enhanced sampling molecular dynamics (MD) simulations. Although progress has been made with data-driven and intuition-based approaches, optimal CVs remain system-specific. Meanwhile, simple geometric descriptors are still widely used due to their transferability. A promising, yet under-explored, candidate for a more effici...
Schulze, M.
•
Khakhula, T.
•
Piasentin, N.
•
Aureli, S.
...•
Gervasio, F. L.
biorxiv
Tue Jul 01 2025
Micron-scale protein transport along microtubules by kinesin-driven shepherding
Microtubule-based long-distance transport in eukaryotic cells typically involves the binding of cargo to motors such as highly processive kinesins for unidirectional transport. An open question is whether long-distance transport can occur by mechanisms that do not require specific motor-cargo interactions and high processivity. In addition to conventional cargo such as vesicles, kinesin also shutt...
Farhadi, L.
•
Fiorenza, S.
•
Wijeratne, S.
•
Nakos, K.
...•
Betterton, M.