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July 3rd, 2025
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neuroscience
biorxiv

A single NPFR neuropeptide F receptor neuron that regulates thirst behaviors in Drosophila

Ramirez, D. O.Open in Google Scholar•Wang, B. P.Open in Google Scholar•Landayan, D.Open in Google Scholar•Kaur, G.Open in Google Scholar•Wolf, F. W.Open in Google Scholar

Thirst is a strongly motivated internal state that is represented in central brain circuits that are only partially understood. Water seeking is a discrete step of the thirst behavioral sequence that is amenable to uncovering the mechanisms for motivational properties such as goal-oriented behavior, value encoding, and behavioral competition. In Drosophila water seeking is regulated by the NPY-like neuropeptide NPF, however the circuitry for NPF-dependent water seeking is unknown. To uncover the downstream circuitry, we identified the NPF receptor NPFR and the neurons it is expressed in as being acutely critical for thirsty water seeking. Refinement of the NPFR pattern uncovered a role for a single neuron, the L1-l, in promoting thirsty water seeking. The L1-l neuron increases its activity in thirsty flies and is involved in the regulation of dopaminergic neurons in long-term memory formation. Thus, NPFR and its ligand NPF, already known for its role in feeding behavior, are also important for a second ingestive behavior.

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