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July 4th, 2025
Version: 1
Instituto de Nanociencia y Materiales de Aragon
bioengineering
biorxiv

MagPiezo: a Magnetogenetic Platform for Remote Activation of Endogenous Piezo1 Channels in Endothelial Cells

Del Sol-Fernandez, S.Open in Google Scholar•De Simone, M.Open in Google Scholar•Fernandez-Afonso, Y.Open in Google Scholar•Garcia-Gonzalez, D.Open in Google Scholar•Martinez-Vicente, P.Open in Google Scholar•van Zanten, T. S.Open in Google Scholar•Fratila, R. M.Open in Google Scholar•Moros, M.Open in Google Scholar

This work introduces MagPiezo, a novel magnetogenetic platform designed to enable remote and targeted manipulation of endogenous Piezo1 channels without genetic modification. MagPiezo integrates a custom-built magnetic applicator and 19 nm octahedral mixed ferrite nanoparticles (MFs) with enhanced magnetocrystalline anisotropy, engineered to selectively target Piezo1 channels. The application of a low intensity (< 40 mT), low frequency (1 Hz) magnetic field is sufficient to generate wireless torque forces in the piconewton range capable of gating Piezo1 channels in vitro. Remarkably, applying MagPiezo for short times (< 1 h) induces robust responses in endothelial cells, including calcium influx, cytoskeletal reorganization and transcriptional activation. Unlike other magnetogenetic tools, MagPiezo avoids the need to assemble the magnetic nanoparticles (MNPs) into bigger beads to reach the opening threshold and does not need to genetically engineer Piezo1 channels. Moreover, it uses low magnetic field intensities and frequencies, enhancing its potential for in vivo translation. Therefore, MagPiezo is a powerful platform for manipulating Piezo1 channels and dissecting mechanotransduction events in vascular biology, paving the way for new strategies to modulate mechanoreceptors both in vitro and in vivo.

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