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July 17th, 2025
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Georgia Institute of Technology
biophysics
biorxiv

Cell-substrate friction controls biofilm development

Pokhrel, A.Open in Google Scholar•Copeland, R.Open in Google Scholar•Hejri, M.Open in Google Scholar•Belpaire, T. E. R.Open in Google Scholar•Steinbach, G.Open in Google Scholar•Ng, S. L.Open in Google Scholar•Hammer, B. K.Open in Google Scholar•Yunker, P. J.Open in Google Scholar

Bacteria often live in biofilms, surface-attached communities that can form on nearly any surface, from coarse sands to smooth glass. It is often hypothesized that cell-substrate friction can impact biofilm growth and development on these disparate surfaces. However, the experimental difficulty in measuring the friction between a biofilm and its surface has limited our understanding of how friction forces impact emergent colony-level behaviors and morphologies. Here, we demonstrate that increasing cell-substrate friction increases the biofilm contact angle, which, in turn, decreases the horizontal biofilm range expansion rate. We first used individual-based simulations to isolate the impact of friction. In this simple model, we found that increasing friction increases biofilm contact angle, and, in turn, that the contact angle emerges from a simple dynamic force balance between cell-substrate friction, cell-cell steric forces, and cell-cell adhesion, reminiscent of Young\'s equation for sessile liquid drops. We developed an approach to directly measure the friction between bacterial colonies and agar surfaces, and found that the friction between the substrate and the biofilm is higher on substrates made with higher agar weight percentage. Additionally, we observed that biofilm contact angles increase with friction, following a Young-equation-like balance. Finally, we found that contact angle increases with agar percentage for a wide range of bacteria, suggesting that the biophysical impact of friction may play a role for a wide array of microbes.

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