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July 18th, 2025
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UCL
neuroscience
biorxiv

Functional Dissociation of Layers 2/3 and 5 in the Primary Visual Cortex

Sit, T. P. H.Open in Google Scholar•Bimbard, C.Open in Google Scholar•Lebedeva, A.Open in Google Scholar•Carandini, M.Open in Google Scholar•Coen, P.Open in Google Scholar•Harris, K. D.Open in Google Scholar

The columnar hypothesis holds that neurons in a single cortical column process similar types of information. Here we show, however, that the way mouse primary visual cortex integrates visual and non-visual information differs fundamentally between layers. We used large-scale two-photon calcium imaging and Neuropixels recordings to compare population activity across layers in awake mice. Layer 2/3 activity is more strongly modulated by visual stimuli, whereas layer 5 activity is more strongly modulated by the animal's movement. Furthermore, movement has opposite effects on population synchrony in the two layers: it desynchronizes the spontaneous oscillations of layer 2/3 while synchronizing layer 5 activity. The geometry of population activity also differs, with spontaneous activity in layer 2/3 being lower-dimensional and more dominated by a single coding dimension than in layer 5, particularly during rest. These results suggest a division of labor, where superficial layers generate a robust sensory representation for downstream cortical processing, while deep layers integrate sensory signals with behavioral context to inform motor output.

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