2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
September 2nd, 2025
Version: 3
University of Wisconsin-Madison
evolutionary biology
bioRxiv

A model for background selection in non-equilibrium populations

Barroso, G. V.Open in Google Scholar•Ragsdale, A.Open in Google Scholar

In many taxa, levels of genetic diversity are observed to vary along their genome. The framework of background selection models this variation in terms of linkage to constrained sites, and recent applications have been able to explain a large portion of the variation in human genomes. However, these studies have also yielded conflicting results, stemming from two key limitations. First, existing models are inaccurate in the most critical region of parameter space (Nes~-1), where the reduction in diversity is sharpest. And second, they assume a constant population size over time. Here, we develop predictions for diversity under background selection based on the Hill-Robertson system of two-locus statistics, which allows for population size changes. We treat the joint effect of multiple selected loci independently, but we show that interference among them is well captured through local rescaling of mutation, recombination and selection in an iterative procedure that converges quickly. We further accommodate existing background selection theory to non-equilibrium demography, bridging the gap between weak and strong selection. Simulations show that our predictions are accurate over the entire range of selection coefficients. We characterize the temporal dynamics of linked selection under population size changes and demonstrate that patterns of diversity can be misinterpreted by other models. Specifically, biases due to the incorrect assumption of equilibrium carry over to downstream inferences of the distribution of fitness effects and deleterious mutation rate. Jointly modeling demography and linked selection therefore improves our understanding of the genomic landscape of diversity, which will help refine inferences of linked selection in humans and other species.

Similar Papers

bioRxiv
Thu Sep 04 2025
Escherichia coli deletes in vivo the same domains from a double-mutant leucyl-tRNA synthetase gene that were deleted in vitro to make the LeuAC urzyme
The Last Universal Common Ancestor (LUCA) lived about 4.2 billion years ago and had a nearly modern metabolism. Genes and proteins must therefore have achieved modern lengths during a time comparable to the error in that date. How did that happen? We show here that E coli transformed with a double mutant full-length Leucyl-tRNA synthetase (LeuRS) produces discrete sets of shorter genes. These lack...
Tang, G. Q.
•
Carter, C. W.
bioRxiv
Wed Sep 03 2025
Genomic signatures of local adaptation across parasitised cricket populations
Host-parasite interactions are predicted to exhibit geographic heterogeneity, creating the opportunity for local adaptation. This is difficult to detect because it requires knowledge of selection pressures and fitness consequences of phenotypic variation. We explored the adaptive landscape of Hawaiian cricket populations which are parasitised by larvae of a fly that targets singing male crickets, ...
Rayner, J. G.
•
Yusuf, L.
•
Zhang, R.
•
Zhang, S.
...•
Bailey, N. W.
bioRxiv
Wed Sep 03 2025
Evolution of increased longevity and slowed ageing in a genus of tropical butterfly
Evolution has given rise to lifespans in extant species ranging from days to centuries. Given that mechanisms of ageing are highly conserved, studying long-lived lineages across the animal kingdom could yield insights relevant for healthy ageing in humans. However, typical models of extended lifespan often live for decades, making them impractical for longitudinal studies. Ideal model systems woul...
Foley, J.
•
McPherson, J.
•
Roger, M.
•
Batista, C.
...•
Montgomery, S. H.
bioRxiv
Tue Sep 02 2025
Inter-individual gene expression variability implies stable regulation of brain-biased genes across organs
Phenotypic variation among individuals plays a key role in evolution, since variation provides the material on which natural selection can act. One important link between genetic and phenotypic variation is gene expression. As for other phenotypes, the range of accessible expression variation is limited and biased by different evolutionary and developmental constraints. Gene expression variability...
Bucao, C. F.
•
Aletti, C.
•
Laverre, A.
•
Moretti, S.
...•
Robinson-Rechavi, M.
bioRxiv
Tue Sep 02 2025
Bayesian inference of introgression between sister lineages using genomic data
Inference of interspecific gene flow using genomic data is important to reliable reconstruction of species phylogenies and to our understanding of the speciation process. Gene flow is harder to detect if it involves sister lineages than nonsisters; for example, most heuristic methods based on data summaries are unable to infer gene flow between sisters. Likelihood-based methods can identify introg...
Yang, Z.
•
Jiao, X.
•
Cheng, S.
•
Zhu, T.
bioRxiv
Tue Sep 02 2025
Context-dependent activation and evolutionary buffering of a mating pheromone in fission yeast
The evolution of mating signals drives reproductive isolation and speciation across diverse lineages. However, how short peptide pheromones, typically subject to strong structural constraints, achieve functional diversification remain unclear. In the fission yeast Schizosaccharomyces pombe, a previously established library of 153 single-amino acid variants of the mating pheromone M-factor was appl...
Seike, T.
•
Sakata, N.
•
Kotani, H.
•
Furusawa, C.
bioRxiv
Tue Sep 02 2025
Reconstructing the 3D genome organization of Neanderthals reveals that chromatin folding shaped phenotypic and sequence divergence
Changes in gene regulation were a major driver of the divergence of archaic hominins (AHs)--Neanderthals and Denisovans--and modern humans (MHs). The three-dimensional (3D) folding of the genome is critical for regulating gene expression; however, its role in recent human evolution has not been explored because the degradation of ancient samples does not permit experimental determination of AH 3D ...
McArthur, E.
•
Rinker, D. C.
•
Cheng, Y.
•
Wang, Q.
...•
Capra, J. A.
bioRxiv
Tue Sep 02 2025
Reductive evolution of the DNA replication machinery in endosymbiotic fungi
The molecular machinery for replicating and repairing DNA accurately is critically important for life and highly conserved across the Tree of Life. Here we show that two major lineages of fungi, Glomeromycotina and Microsporidia, lost DNA polymerase complexes involved in replication and translesion synthesis. Catalytic and non-catalytic subunits of DNA polymerases are co-eliminated, consistent wit...
Dallaire, A.
•
Chancellor, T.
•
Bhattacharya, S.
•
Nishizawa, T.
•
Paszkowski, U.
bioRxiv
Tue Sep 02 2025
SimHumanity: Using SLiM 5.0 to run whole-genome simulations of human evolution
The reconstruction of human evolutionary history has undergone repeated advances, each made possible by methodological innovations. In recent decades, genetic and genomic data played a central role in the reconstruction of major evolutionary events such as the out-of-Africa migration, and genetic simulations of human evolutionary history have come to play a major role in testing more specific hypo...
Haller, B. C.
•
Nelson, C. W.
•
Rodrigues, M. F.
•
Messer, P. W.