2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
September 4th, 2025
Version: 1
University of Western Ontario
neuroscience
bioRxiv

Developing a model of temporomandibular disorder in the common marmoset using nerve growth factor

Holzscherer, E. J.Open in Google Scholar•Kersten, R.Open in Google Scholar•Bertrand, M.Open in Google Scholar•Khokhar, J. Y.Open in Google Scholar•Cairns, B. E.Open in Google Scholar•Pruszynski, J. A.Open in Google Scholar•Seminowicz, D. A.Open in Google Scholar

Developing an animal model that more closely represents the human multidimensional pain experience is an important step towards addressing the current chronic pain crisis. The common marmoset has potential as this model species given its biological, neurological and phylogenetic similarity to humans. Here, we developed a model of myofascial temporomandibular disorder (TMD) in the marmoset by injecting nerve growth factor (NGF) into the superficial masseter. Following injection, animals showed reduced mechanical withdrawal thresholds at 5 g and 10 g doses of NGF and changes in circadian rhythm and feeding initiation following injection of 10 g of NGF. Animals did not show evidence of jaw dysfunction, masticatory alterations, or grimace during novel behavioural assays. The model is transient, with pain resolution occurring approximately 7 days after onset, which allows for repeated testing on the same animal. This same NGF-TMD model has been previously validated in rodents and humans and presents an opportunity for forward and reverse translation to examine mechanisms, develop relevant pain assessment tools, and ultimately test novel treatments for TMD and other musculoskeletal pain conditions.

Similar Papers

bioRxiv
Sat Sep 06 2025
Sensory Compression as a Unifying Principle for Action Chunking and Time Coding in the Brain
The brain seamlessly transforms sensory information into precisely-timed movements, enabling us to type familiar words, play musical instruments, or perform complex motor routines with millisecond precision. This process often involves organizing actions into stereotyped \"chunks\'\'. Intriguingly, brain regions that are critical for action chunking, such as the dorsolateral striatum (DLS), also e...
Kumar, S.
•
Le Cauchois, M. B.
•
Mathis, A.
•
Duncker, L.
...•
Mattar, M. G.
bioRxiv
Sat Sep 06 2025
Spatial grouping modulates the link between individual alpha frequency and temporal integration windows in crowding
Previous research has linked endogenous alpha oscillations (~7-13 Hz) to temporal integration windows in visual perception, with higher individual alpha frequency (IAF) predicting improved temporal segregation. Here, we investigated whether alpha-rhythmic temporal integration is a factor in visual crowding and whether this relationship is mediated by spatial grouping mechanisms. 47 participants pe...
Santoni, A.
•
Ronconi, L.
•
Samaha, J.
bioRxiv
Sat Sep 06 2025
A universal dynamic routing architecture governs the flow of human cortical activity
Behaviour requires distributed neural processing to be flexibly integrated and segregated, which in turn demands that information be dynamically routed across the brain. However, whether a unifying principle governs the routing of neural activity remains unknown. Here, we report that the flow of cortical activity is directed through canonical routing modes that are conserved across individuals, ro...
Vinao-Carl, M.
•
Peach, R. L.
•
Gosztolai, A.
•
David, M. C. B.
...•
Grossman, N.
bioRxiv
Sat Sep 06 2025
HuD controls widespread RNA stability to drive neuronal activity-dependent responses
Neuronal activity shapes brain development and refines synaptic connectivity in part through dynamic changes in gene expression. While activity-regulated transcriptional programs have been extensively characterized, the holistic effects of neuronal activity on the full RNA life cycle remain relatively unexplored. Here, we show that neuronal activity influences multiple stages of RNA metabolism in ...
Duffy, E. E.
•
Patop, I.
•
Kalaora, S.
•
Assad, E. G.
...•
Greenberg, M. E.
bioRxiv
Sat Sep 06 2025
Compositional neural dynamics during reaching
The complex mechanics of the arm make the neural control of reaching inherently posture dependent. Because previous reaching studies confound reach direction with final posture, it remains unknown how neural population dynamics in the motor cortex account for arm posture. Here we address this gap with high-density neural recordings and a reaching task in which the same targets serve as start point...
Kashefi, M.
•
Michaels, J. A.
•
Kersten, R.
•
Lau, J. C.
...•
Pruszynski, J. A.
bioRxiv
Sat Sep 06 2025
Benefits of Music Training for Learning to Read: Evidence from Cortical Tracking of Speech in Children
Musical training has long been argued to boost early phonological and reading abilities. Cortical tracking of speech (CTS) has been proposed as a mechanism for this music-to-literacy transfer. In this study, we examined how musical training shapes CTS in young readers and whether it facilitates literacy benefits. In a sample of 57 children aged 5-9, musical training was linked to enhanced reading ...
Garcia-de-Soria, M. C.
•
Mathias, B.
•
Keitel, A.
•
Klimovich-Gray, A.
bioRxiv
Sat Sep 06 2025
Complementary regulation of memory flexibility and stabilization by dentate gyrus granule cells and mossy cells
Accurate memory formation requires hippocampal spatial representations to balance stability, for later recall, with flexibility, to incorporate new information. The dentate gyrus (DG) is essential to memory formation, but the distinct roles of its excitatory cell types, granule cells (GCs) and mossy cells (MCs), remain unclear. To evaluate how GC and MC activity affect hippocampal output, we recor...
GoodSmith, D.
•
Carson, W. H.
•
Sheffield, M. E.
bioRxiv
Sat Sep 06 2025
Neural Subspaces Encode Sequential Working Memory, but Neural Sequences Do Not
The neural mechanisms of multiple-item working memory are not well understood. In the current study, we address two competing hypotheses about the neural basis of sequential working memory: neural subspaces versus neural sequences. Using broadband MEG data from human participants, we applied dimensionality reduction and multivariate decoding techniques to test whether sequential items are maintain...
Santo-Angles, A.
•
Yang, J.
•
Zhou, Y.
•
Chu, W. K. H.
...•
Sreenivasan, K. K.
bioRxiv
Sat Sep 06 2025
The human factor: development and characterization of a scalable calcium imaging assay using human iPSC-derived neurons
Neuroscience drug discovery is challenged by the brain\'s structural and cell-type complexity, which is difficult to model in cellular systems compatible with high-throughput screening methods. Calcium oscillation assays, that harness neurons\' intrinsic capability to develop functional neural networks in cell culture, are currently the closest cellular models with a relevant functional endpoint t...
Haustein, M. D.
•
Deymier, C.
•
Schlienger, S.
•
Lexcellent-Bissler, L.
...•
Portmann, T.
bioRxiv
Sat Sep 06 2025
Fast spiking interneurons autonomously generate fast gamma oscillations in the medial entorhinal cortex with excitation strength tuning ING-PING transitions
Gamma oscillations (40-140 Hz) play a fundamental role in neural coordination, facilitating communication and cognitive functions in the medial entorhinal cortex (mEC). While previous studies suggest that pyramidal-interneuron network gamma (PING) and interneuron network gamma (ING) mechanisms contribute to these oscillations, the precise role of inhibitory circuits remains unclear. Using optogene...
Williams, B.
•
Vedururu Srinivas, A.
•
Baravalle, R.
•
Fernandez, F. R.
...•
White, J. A.