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September 4th, 2025
Version: 10
Icahn School of Medicine at Mount Sinai
cell biology
bioRxiv

A multipotent cell type from term human placenta

Vadakke-Madathil, S.Open in Google Scholar•Bouhamida, E.Open in Google Scholar•Wang, B.Open in Google Scholar•Mathiyalagan, P.Open in Google Scholar•Ayyamperumal, P.Open in Google Scholar•Khan, A.Open in Google Scholar•Oniskey, M.Open in Google Scholar•Santos-Gallego, C.Open in Google Scholar•Hadley, M.Open in Google Scholar•Croft, L.Open in Google Scholaret al.

We identify a population of multipotent CDX2; cells from term human placentas with clonal expansion, migratory capacity, and immune-privileged transcriptional profiles. Isolated from 180 healthy pregnancies, these cells differentiate into cardiomyocyte and vascular lineages in vitro and in vivo. Single-cell RNA sequencing uncovers distinct cardiogenic and vasculogenic subpopulations, along with immune-modulatory and chemotactic programs, providing a blueprint for precision-guided cardiovascular cell therapy. In a NOD/SCID myocardial infarction model, CDX2; cells restore cardiac function, and clonal propagation preserves their cardiovascular differentiation potential. These findings position placental CDX2; cells as an ethically accessible, regenerative platform for targeted treatment of cardiovascular disease.

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