2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
April 1st, 2025
Version: 1
Korea University
bioengineering
biorxiv

Scalable, high-throughput isolation of extracellular vesicles using electrokinetic-assisted mesh filtration

Lee, K.Open in Google Scholar•Bae, M.Open in Google Scholar•Kim, Y.Open in Google Scholar•Jeon, S.Open in Google Scholar•Kang, S.Open in Google Scholar•Rhee, W.Open in Google Scholar•SHIN, S.Open in Google Scholar

As extracellular vesicles (EVs) are increasingly recognized for their superior functions for therapeutics, the need for large-scale EV isolation technology is becoming more critical for clinical and industrial applications. Most existing EV isolation methods are optimized for small-scale laboratory samples, limiting their efficiency and scalability for large-scale production. Here, an electrokinetic-assisted filtration system (ExoFilter), which introduces charge interaction into physical mesh flow filtration, is proposed as a new candidate to address the challenges of scalable EV isolation. The hybrid filtration system demonstrates outstanding high-throughput EV isolation performance (a flux of ~750 mL/min) using only a coarse physical filter, by electrokinetically arresting EVs flowing through the filter lattice. Furthermore, the recovery efficiency of ExoFilter, analyzed based on the ELISA results, was found to be approximately 98%, demonstrating the filter\'s exceptional efficiency in EV isolation. Additionally, ExoFilter enables the rapid isolation of EVs from small samples as little as 200 uL, facilitating quick and easy blood-based EV research. Furthermore, low-molecular-weight albumin from plasma samples was effectively removed. The high-throughput and high-efficiency characteristics of ExoFilter make it well-suited for scalable EV production, offering greater convenience for various clinical applications.

Similar Papers

biorxiv
Wed Apr 02 2025
Strengthening Antisense Oligonucleotide-Mediated Anti-Tumor Immunity via Metal-Organic Framework Nanoparticles
Overexpression of checkpoint proteins, such as programmed death ligand one (PD-L1), prevents immune recognition and enables cancer growth. Current monoclonal antibodies that block PD-L1 tend to be fragile, unable to penetrate tumors, and target cancer at later stages, thus leading to inconsistent patient outcomes. Antisense oligonucleotides (ASOs) provide an alternative to decrease PD-L1 expressio...
Nowak, J. A.
•
Cho, E.
•
Davis, M. A.
•
Zheng, S.
...•
Teplensky, M. H.
biorxiv
Wed Apr 02 2025
Compact lens-based dual-channel adaptive optics scanning laser ophthalmoscopy for in-vivo three-dimensional retinal imaging in mice
Adaptive optics (AO) has been instrumental in ophthalmic imaging, by correcting wavefront aberrations in ocular optics and achieving diffraction-limited resolution. Current state-of-the-art AO retinal imaging systems use mirror-based optics to avoid surface reflection and chromatic aberrations, requiring a large system footprint with long focal length spherical mirrors. Here we report a compact re...
Li, Z.
•
Mary, S.
•
Johnson, T. V.
•
Yi, J.
biorxiv
Wed Apr 02 2025
Toward De Novo Protein Design from Natural Language
De novo protein design represents a fundamental pursuit in protein engineering, yet current deep learning approaches remain constrained by their narrow design scope. Here we present Pinal, a large-scale frontier framework comprising 16 billion parameters and trained on 1.7 billion protein-text pairs, that bridges natural language understanding with protein design space, translating human design in...
Dai, F.
•
You, S.
•
Wang, C.
•
Fan, Y.
...•
Yuan, F.
biorxiv
Wed Apr 02 2025
Functional Neural Connectivity of the Mouse Brain using Photoacoustic Ultrasound Imaging
Brain connectomes are insightful models that describe the connectivity of different regions throughout the brain. These connectomes are traditionally generated through correlation of oxygen data from Functional magnetic resonance imaging (fMRI) scans. Photoacoustic ultrasound (PAU) can also detect oxygenation levels while being more accessible and cost effective than fMRI. We propose the use of PA...
Clark, I. H.
•
Heinmiller, A.
•
Zhu, W.
•
Zhu, X.-H.
...•
Chen, W.
biorxiv
Wed Apr 02 2025
Rapid optimization of protein function in mammalian cells via microbe-independent deep assembly and screening
Random mutagenesis and deep mutational scanning (DMS) are widely used to optimize proteins by oversampling large libraries in microbial cells, selecting cells expressing favorable variants, and sequencing to identify enriched variants. However, these methods are slow and costly, require effort to establish selection methods for a given protein function, and do not yield data on lower-performing va...
Wu, Y.
•
Wang, P.
•
Liu, L. X.
•
Gao, C.
...•
Lin, M. Z.
biorxiv
Wed Apr 02 2025
Light-field deep learning enables high-throughput, scattering-mitigated calcium imaging
Light field microscopy (LFM) enables volumetric, high throughput functional imaging. However, the computational burden and vulnerability to scattering limit LFM's application to neuroscience. We present a light-field strategy for volumetric, scattering-mitigated neural circuit activity monitoring. A physics-based deep neural network, LNet, is trained with two-photon volumes and one-photon light fi...
Howe, C. L.
•
Zhao, K. L. Y.
•
Verinaz-Jadan, H.
•
Song, P.
...•
Foust, A. J.
biorxiv
Wed Apr 02 2025
Transcranial Functional Ultrasound Imaging Detects Focused Ultrasound Neuromodulation Induced Hemodynamic Changes In Vivo
Background: Focused ultrasound (FUS) is an emerging non-invasive technique for neuromodulation in the central nervous system (CNS). Functional ultrasound imaging (fUSI) leverages ultrafast Power Doppler Imaging (PDI) to detect changes in cerebral blood volume (CBV), which correlate well with neuronal activity and thus hold promise to monitor brain responses to FUS. Objective: Investigate the immed...
Bendig, J.
•
Aurup, C.
•
Blackman, S. G.
•
McCune, E. P.
...•
Konofagou, E. E.
biorxiv
Tue Apr 01 2025
Sustained neurotrophic factor cotreatment enhances donor and host retinal ganglion cell survival in mice
Retinal ganglion cells (RGCs) lack regenerative capacity in mammals, and their degeneration in glaucoma leads to irreversible blindness. Autologous and allogeneic RGC replacement with stem cell-derived neurons has been established as a promising strategy for vision restoration, but it has been limited by relatively low (<1%) survival rates of donor cells in the hostile microenvironment of a diseas...
Soucy, J. R.
•
Oswald, J.
•
Kriukov, E.
•
Phay, M.
...•
Baranov, P.
biorxiv
Tue Apr 01 2025
Rapid optimization of protein function in mammalian cells via microbe-independent deep assembly and screening
Random mutagenesis and deep mutational scanning (DMS) are widely used to optimize proteins by oversampling large libraries in microbial cells, selecting cells expressing favorable variants, and sequencing to identify enriched variants. However, these methods are slow and costly, require effort to establish selection methods for a given protein function, and do not yield data on lower-performing va...
Wu, Y.
•
Wang, P.
•
Liu, L. X.
•
Gao, C.
...•
Lin, M. Z.
biorxiv
Tue Apr 01 2025
Elevated hydrostatic pressure acts via piezo-1 to destabilise VE-cadherin junctions: an endothelium-on-chip study
Despite the effects of shear stress on endothelial biology having been extensively researched, the effects of hydrostatic vascular pressure at extremely low shear stresses have been largely ignored. In the current study, we employ a microfluidic organ-on-chip platform to elucidate the time and shear stress dependent effects of elevated hydrostatic pressure on endothelial junctional perturbations. ...
Bathrinarayanan, P. V.
•
Abadie, T.
•
Esteban, P. P.
•
Vigolo, D.
...•
Grover, L. M.