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April 10th, 2025
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Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agricultural Sciences in Weifang, Shandong Provincial Key Laborat
genomics
biorxiv

Two telomere-to-telomere, gap-free genome assemblies and comparisons revealed the conserved key genes associated with sugar accumulation in Rubus genus

Li, X.Open in Google Scholar•Han, X.Open in Google Scholar•Liu, S.Open in Google Scholar•Zhang, Q.Open in Google Scholar•Guan, J.Open in Google Scholar•Tang, Y.Open in Google Scholar•Zhang, M.Open in Google Scholar•Lian, H.Open in Google Scholar•Xu, P.Open in Google Scholar•Zheng, M.Open in Google Scholaret al.

Two telomere-to-telomere, gap-free genome for Rubus species were sequenced and assembled by using an integrative approach that combined ultra-long reads from Oxford Nanopore Technology (ONT), PacBio high-fidelity (HiFi) long reads, Illumina paired-end short reads, and High-throughput Chromosome Conformation Capture (Hi-C) data, resulted in the first gap-free reference genomes of the Rubus genus. By comparative genomics and transcriptomics, we revealed the conserved key genes associated with sugar accumulation in Rubus genus, providing candidate target sites for improving fruit quality in Rubus. We also established a database for documenting all the genomic, transcriptomic, and phenotypic data generated in this study.

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