2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
April 21st, 2025
Version: 2
Universite Paris Cite
biophysics
biorxiv

Influencing Iron Homeostasis by Transition Metal Complexes Interacting with a messenger-RNA Stem Loop in the Iron Regulating Element

Hognon, C.Open in Google Scholar•Froux, A.Open in Google Scholar•Grandemange, S.Open in Google Scholar•MONARI, A.Open in Google Scholar

The maintaining of the precise regulation of iron homeostasis is fundamental to assure cells viability. In this context the interplay between the messenger RNA Iron Response element (IRE) and the iron response protein 1 (IRP1) is crucial to regulate the expression of ferritin and hence the level of labile free iron pool. We have shown, using a combination of molecular modeling and experimental techniques, that tris bipyridine iron complexes (AIM3) interact specifically with the IRE RNA stem-loop in solution, without inducing noticeable structural deformations. Furthermore, we have also shown that, at a cellular level, this interaction may be traced back to the downregulation of ferritin translation, probably due to the stabilization of the IRE stem-loops thus favoring its binding to IRP1 or by inhibiting the downstream recruitment of ribosomal subunits.

Similar Papers

biorxiv
Mon Apr 21 2025
Dynamical properties of chromatin provide insights into key folding principles
Even though the three-dimensional static organization of chromatin is highly studied, chromatin is a dynamic structure, and time-dependent changes are crucial for biological function. While it is known that both intra-chromatin interaction and loop extrusion are crucial to understanding chromatin organization, what is their respective role in deciding the nature of spatial and temporal organizatio...
Kadam, S.
•
Sahoo, S.
•
Kumar, P. B. S.
•
Padinhateeri, R.
biorxiv
Mon Apr 21 2025
Protein Electrostatic Properties are Fine-Tuned Through Evolution
Protein ionization states provide electrostatic forces to modulate protein structure, stability, solubility, and function. Until now, predicting ionization states and understanding protein electrostatics have relied on structural information. Here we demonstrate that primary sequence alone enables remarkably accurate pKa predictions through KaML-ESM, a model that leverages evolutionary representat...
Shen, M.
•
Dayhoff, G. W.
•
Shen, J.
biorxiv
Mon Apr 21 2025
A Non-Canonical Interface of SNX9 PX Domain Selectively Sequesters PI(3,4)P2 Lipids, Protecting Them from Hydrolysis
Plasma membrane remodeling processes are tightly regulated by the spatiotemporal distribution and dynamic conversion of phosphoinositidyl lipids (PIPs). This regulation is controlled by the recruitment of proteins such as sorting nexin 9 (SNX9), a key mediator of late-stage endocytosis and macropinocytosis. Using live cell imaging, in vitro reconstitution assays, and molecular dynamics simulations...
Beiter, J.
•
Sung, C.-J.
•
Lin, S.-S.
•
DeVuono, N.
...•
Tsai, F.-C.
biorxiv
Mon Apr 21 2025
Mechanism of USP21 autoinhibition and histone H2AK119 deubiquitination
Monoubiquitinated histone H2A lysine 119 (H2AK119ub) is a signature modification associated with transcriptional silencing and heterochromatin formation. Ubiquitin-specific protease 21 (USP21), one of four major deubiquitinating enzymes (DUBs) that target H2AK119ub, plays critical roles in diverse cellular processes. The molecular mechanisms by which USP21 specifically deubiquitinates H2AK119ub an...
Rahman, S.
•
Morgan, M. T.
•
Hicks, C. W.
•
Gwizdala, A.
•
Wolberger, C.
biorxiv
Mon Apr 21 2025
A Beauty Dye Staining (ABDS) - inexpensive marker-ink based open-source alternative for commercial membrane vital dyes
In this paper, we aim to present a new intravital dye, called ABDS, which can be prepared using a marker pen and is useful for eukaryotic cell research. ABDS binds to the cytoplasmic membrane of living cells and can be used for their visualization in biophysical experiments, including Z-stack tomography, cell proliferation monitoring, and adhesion studies. Important properties of ABDS are its avai...
Abelit, A. A.
•
Boytsova, N. A.
•
Kornev, A. A.
•
Yakovleva, L. E.
•
Daniil, S. D.
biorxiv
Mon Apr 21 2025
Synaptic Spine Head Morphodynamics from Graph Grammar Rules for Actin Dynamics
There is a morphodynamic component to synaptic learning by which changes in dendritic spine head size are associated with the strengthening or weakening of the synaptic connection between two neurons, in response to the temporal correlation of local presynaptic and postsynaptic signals. Morphological factors are in turn sculpted by the dynamics of the actin cytoskeleton. We use Dynamical Graph Gra...
Hur, M.
•
Bartol, T.
•
Rangamani, P.
•
Sejnowski, T.
•
Mjolsness, E.
biorxiv
Mon Apr 21 2025
Modeling glioma-induced impairments on the glymphatic system
Altered glymphatic function is observed for many neurological diseases. Glioma, one of the most common brain cancers, is known to have altered fluid dynamics in terms of edema and blood-brain-barrier breakdown, both features potentially impacting the glymphatic function. To study glioma and its fluid dynamics, we propose a flexible mathematical model, including the tumor, the peri-tumoral edema as...
Poulain, A.
•
Riseth, J. N.
•
Emblem, K. E.
•
Mardal, K.-A.
biorxiv
Sun Apr 20 2025
Dynamic Pathway of Guanidine-III Riboswitch Folding Revealed by Single-Molecule FRET: Mg2+-Assisted Preorganization and Ligand-Induced Kinetic Trapping
Riboswitches are structured RNA elements that regulate gene expression by sensing and binding small molecules. The guanidine-III riboswitch, a critical bacterial regulator responding to guanidine toxicity, undergoes precise conformational changes that remain poorly characterized at a dynamic, mechanistic level. In this study, we employed single-molecule Forster Resonance Energy Transfer (smFRET) c...
Jiang, M.
•
Bian, Y.
•
Zou, L.
•
Liu, T.
...•
Pan, H.
biorxiv
Sat Apr 19 2025
From Decay to Rhythm: Sustained Biological Oscillators Require More Than Chemistry Alone
Many biological systems exhibit rhythmic oscillatory dynamics, yet it is shown that chemical reaction systems governed by Markovian dynamics cannot generate long-lasting oscillations. To overcome this limitation, we propose a general mechanism that couples a Markovian system to at least one other degree of freedom, such as a mechanical system, to achieve sustained oscillations with a desired frequ...
Wu, Y.
•
Sun, S.