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April 21st, 2025
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Alferov University
biophysics
biorxiv

A Beauty Dye Staining (ABDS) - inexpensive marker-ink based open-source alternative for commercial membrane vital dyes

Abelit, A. A.Open in Google Scholar•Boytsova, N. A.Open in Google Scholar•Kornev, A. A.Open in Google Scholar•Yakovleva, L. E.Open in Google Scholar•Daniil, S. D.Open in Google Scholar

In this paper, we aim to present a new intravital dye, called ABDS, which can be prepared using a marker pen and is useful for eukaryotic cell research. ABDS binds to the cytoplasmic membrane of living cells and can be used for their visualization in biophysical experiments, including Z-stack tomography, cell proliferation monitoring, and adhesion studies. Important properties of ABDS are its availability, bright stable fluorescence, manufacturing simplicity, and safety for living cells in vitro. The paper includes a method for preparing ABDS, a data set with its characteristics, as well as examples of ABDS use in cells investigation.

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