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June 3rd, 2025
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Mack Planck Institute of Molecular Plant Physiology
plant biology
biorxiv

Diversification of the "EDVID" packing motif underpins structural and functional variation in plant NLR coiled-coil domains

Sulkowski, O.Open in Google Scholar•Ovodova, A.Open in Google Scholar•Leisse, A.Open in Google Scholar•Gögelein, K.Open in Google Scholar•Förderer, A.Open in Google Scholar

{middle dot} Nucleotide-binding leucine-rich repeat receptors (NLRs) are critical in plant immunity and display remarkable allelic diversity. Coiled-coiled NLRs (CC-NLRs) are the most widespread group of these receptors found across flowering and non-flowering plants. {middle dot} Here we investigate the sequence conservation and functional variation of the conserved EDVID motif found in the 3-helix of the cell death inducing CC domain of plant NLRs. We analyse our findings in context of published protein structures and structure prediction. {middle dot} We find that the conserved EDVID motif can serve as a predictor of canonical CC-NLR function and oligomeric assembly. {middle dot} We also find that the EDVID motif is accompanied by preceding acidic residues in certain CC-NLRs with homology to the Arabidopsis CC-NLR RPP8. The appearance of this so-called preEDVID motif across the phylogeny of flowering plants and its contribution to the CC-NLR function underpins the structural diversity across NLRs with EDVID motif. {middle dot} We further show that CC-NLRs exist that have lost the EDVID motif sequence and function suggesting that this subgroup, previously referred to as CCG10-NLRs, functions in a different manner from the canonical mechanism. {middle dot} We find that acidic residues located to the 3-helix of the helper NLR NRG1.1 are linked to NRG1.1 cell death inducing activity.

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