2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
July 17th, 2025
Version: 3
Sichuan University
microbiology
biorxiv

Multi-omics investigation of spontaneous T2DM macaque emphasizes gut microbiota could up-regulate the absorption of excess palmitic acid in the T2DM progression

Liu, X.Open in Google Scholar•Xie, Y.Open in Google Scholar•Yang, S.Open in Google Scholar•Jiang, C.Open in Google Scholar•Shang, K.Open in Google Scholar•Luo, J.Open in Google Scholar•Zhang, L.Open in Google Scholar•Hu, G.Open in Google Scholar•Liu, Q.Open in Google Scholar•Yue, B.Open in Google Scholaret al.

Although gut microbiota and lipid metabolites have been suggested to be closely associated with type 2 diabetes mellitus (T2DM), the interactions between gut microbiota, lipid metabolites and the host in T2DM development remains unclear. Rhesus macaques may be the best animal model to investigate these relationships given their spontaneous development of T2DM. We identified eight spontaneous T2DM macaques and conducted a comprehensive study investigating the relationships using multi-omics sequencing technology. Our results from 16S rRNA, metagenome, metabolome and transcriptome analyses identified that gut microbiota imbalance, tryptophan metabolism and fatty acid beta oxidation disorders, long-chain fatty acid (LCFA) accumulation, and inflammation occurred in T2DM macaques. We verified the accumulation of palmitic acid (PA) and activation of inflammation in T2DM macaques. Importantly, mice transplanted with spontaneous T2DM macaque fecal microbiota and fed a high PA diet developed prediabetes within 120 days. We determined that gut microbiota mediated the absorption of excess PA in the ileum, resulting in the accumulation of PA in the serum consequently leading to T2DM in mice. In particular, we demonstrated that the specific microbiota composition was probably involved in the process. This study provides new insight into interactions between microbiota and metabolites and confirms causative effect of gut microbiota on T2DM development.

Similar Papers

biorxiv
Thu Jul 17 2025
Establishing Essential Oil Stewardship Through the Case of Rosemary and Thyme Oils Against Staphylococcus aureus
Essential oils (EOs) have long been studied for their antimicrobial properties, yet most investigations rely on simplistic models, limited strain panels, and anecdotal interpretations - failing to meet the standards expected of modern anti-infective agents. Advancing beyond this tradition, we implement a framework developed over several years of systematic investigation. Using this approach, we as...
Brozyna, M.
•
Stepnicka, Z.
•
Kapczynska, K.
•
Dudek, B.
...•
Junka, A. F.
biorxiv
Thu Jul 17 2025
Genome-guided manipulation of regulators of morphogenesis in a C. albicans strain with low virulence is not sufficient to trigger a high-virulence phenotype
As a member of the microbiome, C. albicans colonizes the oral cavity and other mucosal surfaces of the human body. While commensalism is tightly controlled by the host immune system, the fungal determinants enabling the fungus to colonize the host mucosa without causing tissue damage and inflammation remain less clear. In search of genetic determinants that may underly the commensal lifestyle of t...
Frois-Martins, R.
•
Mertens, S.
•
Tran, V. D. T.
•
Maufrais, C.
...•
LeibundGut-Landmann, S.
biorxiv
Thu Jul 17 2025
A Simple and Cost-Effective Protocol for Enriching 'Ca. Saccharimonadia' from Human Saliva to Enable Efficient Genome Sequencing
Candidatus Saccharimonadia, a lineage within the Candidate Phyla Radiation (CPR), is characterized by small cell size, reduced genomes, and an epibiotic lifestyle dependent on bacterial hosts. Despite their presence in diverse environments, including the human oral microbiota, their role in human health remains largely unexplored. Observational studies have linked increased Ca. Saccharimonadia abu...
Bonaiti, C.
•
Panelli, S.
•
Bettoni, G.
•
Sterzi, L.
...•
Comandatore, F.
biorxiv
Thu Jul 17 2025
Antimicrobial-producing Bacteria from Fish Epidermal Mucus Alter the Fish Epidermal Bacterial Flora and Host Resistance to Infection
The emergence of antimicrobial-resistant bacteria in aquaculture has raised the need for alternative strategies to control fish infections. Antimicrobial-producing bacteria have been explored as probiotics or biocontrol agents, but their mechanisms of action and impact on host-associated microbiota remain poorly understood. Here, we identified Pseudomonas mosselii KH-ZF1, a bacterium isolated from...
Nakatani, H.
•
Suetake, N.
•
Hori, K.
biorxiv
Thu Jul 17 2025
WITHDRAWN: Changes in endophytic microbial diversity and function in the root system affect the growth of continuously planted Casuarina equisetifolia
Continuous planting barrier is an important limiting factor for the growth of Casuarina equisetifolia (C. equisetifolia), an ecological and economic tree varieties, but its underlying microbial mechanism has not been fully elucidated. This study revealed microbial-driven barriers by examining the impact of continuous planting on root endophyte communities in C. equisetifolia. The results showed th...
Hong, L.
•
Wang, Y.
•
Li, M.
•
Li, J.
...•
Wu, Z.
biorxiv
Thu Jul 17 2025
Spatiotemporal distribution and species diversity of pathogenic Vibrios in estuarine recreational waters of southeast Louisiana
Vibrio bacteria occur naturally in brackish water and can cause illnesses through recreational water exposure. Vibrio infections have shown a notable increase in recent years worldwide. In this study, bacterial culture and molecular methods were used to assess the prevalence and diversity of Vibrio species in the estuary of Lake Pontchartrain, the second largest inland brackish body in the United ...
Nelson, A.
•
Cedraz, F.
•
Vigil, K.
•
Alarcon, J.
•
Aw, T.
biorxiv
Thu Jul 17 2025
Beyond translation initiation: dual regulatory interactions with phosphorylated and non-phosphorylated LeishIF4E1
Translation initiation in eukaryotes begins with the assembly of the cap-binding eIF4F complex at the 5 ends of mRNAs, through eIF4E. Among the six orthologs of eIF4E in Leishmania, LeishIF4E1 is intriguing, as it does not bind any Leishmania eIF4G. It is expressed in both life-forms, maintaining efficient cap-binding activity for translation initiation, unlike other LeishIF4Es. We identified two ...
Pullaihagari, D.
•
Baron, N.
•
Purushostam, R.
•
Kamus-Elimeleh, D.
...•
Shapira, M.
biorxiv
Thu Jul 17 2025
N-acetyltransferase 10 promotes mRNA stability of immune response factors to modulate Zika virus infection
Zika virus (ZIKV) is a re-emerging mosquito-borne flavivirus that poses serious risks to human health. In previous work, we used RNA immunoprecipitation and mass spectrometry to show that more than 30 distinct RNA modifications, or chemical moieties, were present on the RNA genome of ZIKV. Among these, N4-acetylcytosine (ac4C) was one of the most abundant modifications. In this study, we investiga...
Gianola, S.
•
Lane, N.
•
Kaytes, K.
•
Pager, C. T.
biorxiv
Thu Jul 17 2025
Applying 3D correlative structured illumination microscopy and X-ray tomography to characterise herpes simplex virus-1 morphogenesis
To date, the characterisation of HSV-1 mutants in the study of virus assembly has been limited to transmission electron microscopy (TEM) without the addition of correlative light microscopy to identify fluorescently labelled viral proteins. In addition, only a small number of mutants are typically used in each study. Herein, a comparative analysis of nine HSV-1 mutants lacking specific structural ...
Nahas, K. L.
•
Connor, V.
•
Wijesinghe, K. J.
•
Barrow, H. G.
...•
Crump, C. M.
biorxiv
Thu Jul 17 2025
Enhancing and stabilizing a high-yield industrial Rhodobacter sphaeroides strain for coenzyme Q10 production
Strengthening high-yield phenotypes while maintaining physiological and genetic stability presents a significant challenge in the improvement of high-yield industrial strains (HIS). Coenzyme Q10 (CoQ10), a crucial quinone electron carrier in the electron transport chain, is widely used in the prevention and treatment of cardiovascular diseases. In this study, the established HIS Rhodobacter sphaer...
Liang, M.
•
He, X.
•
Lv, D.
•
Liu, J.
...•
Tan, G.-Y.