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September 3rd, 2025
Version: 2
Universite Paris-Saclay, UVSQ, INRAE, BREED, 78350 Jouy-en-Josas, France
developmental biology
bioRxiv

Short-term exposure to culture media used in human ART shapes early calcium oscillations in ICSI-fertilized mouse oocytes and impacts adult phenotype

Banrezes, B.Open in Google Scholar•Sainte-Beuve, T.Open in Google Scholar•Frambourg, A.Open in Google Scholar•Jouneau, A.Open in Google Scholar

The influence of culture media used during in vitro fertilization (IVF) on offspring phenotype remains controversial. However, specific effects of short exposure time after fertilization remain underexplored. By evaluating calcium oscillations as a readout of the first response of eggs to their microenvironment, we aim to investigate if early differences correlate with later adult phenotypes. Oocytes fertilized by ICSI were cultured for four hours in three different media (Cook and Vitrolife, used in human IVF, and KSOM, used for mouse embryos). They were either measured for calcium oscillations or transferred into pseudo-pregnant females. After birth, growth curves of pups were measured up to adulthood and various organs weighed. Culture media significantly modulate calciumoscillations during oocyte activation. ICSI-fertilized oocytes cultured in Cook and Vitrolife exhibited fewer oscillations, lower frequency, and reduced variability compared to KSOM. These early differences correlated with long-term developmental outcomes: females from Cook and Vitrolife cultures were heavier throughout growth and had larger adult organ sizes compared to those from KSOM. Brief exposure to media immediately after ICSI shapes calcium dynamics and adult phenotypes. Optimizing embryo culture protocols in assisted reproductive technologies may improve IVF outcomes by modulating metabolic pathways linked to development.

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