2025 Hyper Recent •CC0 1.0 Universal

This work is dedicated to the public domain. No rights reserved.

Access Preprint From Server
September 6th, 2025
Version: 1
Indian Institute of Science Education and Research
developmental biology
bioRxiv

Mitochondrial fission regulates ROS for ventral furrow formation in Drosophila gastrulation

Madan, S.Open in Google Scholar•Chowdhary, S.Open in Google Scholar•Phalke, S.Open in Google Scholar•Mittal, H.Open in Google Scholar•Mavrakis, M.Open in Google Scholar•Rikhy, R.Open in Google Scholar

Mitochondrial fusion and fission are essential for embryogenesis, but the mechanisms by which they regulate morphogenesis are not entirely understood. In this study, we elucidate the role of mitochondrial fission in Drosophila gastrulation. We find that fragmented mitochondria migrate apically in constricting ventral cells in a microtubule and Dorsal/NFkb dependent manner. Reduced mitochondrial fission in Drp1 mutant embryos leads to accumulation of larger mitochondria basally and fewer apical mitochondria. Drp1 mutants show decreased Myosin II, resulting in defective ventral furrow formation. Transcriptomic analysis from Drp1 mutants shows no changes in the Dorsal signaling pathway. Proteomic analysis reveals increased levels of antioxidant enzymes and decreased respiratory complexes, resulting in low ROS levels. Depleting SOD2 in Drp1 mutants restores ROS levels, allowing apical mitochondrial migration, Myosin II increase and proper ventral furrow formation. Our study reveals a significant role for mitochondrial dynamics and activity for apical constriction during gastrulation.

Similar Papers

bioRxiv
Sun Sep 07 2025
DISC1-PML protein interaction for congenital CMV infection-induced cortical neural progenitor deficit: perturbance of host signaling via viral IE1
Congenital CMV infection is the most common perinatal infection, affecting up to 0.5% of infants. This elicits long-term disabilities that include neuropsychiatric manifestations, such as intellectual disability, microcephaly. Despite its high prevalence, the underlying mechanism of how congenitally acquired CMV infection causes brain pathology remains unknown. Here we discovered the molecular int...
Saito, A.
•
Tankou, S.
•
Ishii, K.
•
Sakao-Suzuki, M.
...•
Sawa, A.
bioRxiv
Sun Sep 07 2025
Integrated transcriptomic analysis reveals a metabolically quiescent state and gene expression networks related to intermediate and mature human 8-cell stage embryo resembling cells in vitro
Human early development is challenging to study due to limited samples and cell numbers. The emergence of 8-cell stage embryo-like cells (8CLCs) offers new opportunities to understand embryonic genome activation (EGA) in humans. Our research compares and characterizes 8CLCs from various stem cell-based systems to determine how well these models reflect human early embryonic development. Using sing...
Paloviita, P.
•
Nykanen, S.
•
Vuoristo, S.
bioRxiv
Sun Sep 07 2025
A Hedgehog-Induced Homeodomain Cascade Couples Progenitor Identity to Neuronal Diversification
How a limited progenitor pool generates neuronal diversity remains a longstanding question. In the Drosophila lamina, a single progenitor type generates five lamina neuron subtypes, second-order neurons downstream of photoreceptors. Here, we identify a pan-class homeodomain transcription factor (HDTF), induced by Hedgehog signaling in progenitors and maintained across all five subtypes, that drive...
Xu, C.
•
Sastry, R.
•
Newstein, P.
•
Delgado, R.
...•
Doe, C.
bioRxiv
Sun Sep 07 2025
Tartrate-resistant acid phosphatase (TRAP/ACP5) sex-specifically regulates bone maintenance in old mice, but the anabolic effects of mechanical loading is regulated in a sex-independent way
Tartrate-resistant acid phosphatase (TRAP/ACP5), primarily known as an osteoclast marker, has emerged as a critical regulator of skeletal integrity, regulating sex-specific bone growth, and bones response to mechanical load in young adult male mice. In this study, we investigated the sex-specific roles of TRAP in bone structure and response to mechanical stimuli in old (19-month-old) wild-type (WT...
Rathod, B.
•
Samvelyan, J.
•
Gustafsson, N.
•
Liszka, A.
...•
Windahl, S. H.
bioRxiv
Sun Sep 07 2025
CUT&TIME captures the history of open chromatin indeveloping neurons
Chromatin structure plays a central role in defining cell identity by regulating gene expression. During development, shifts in chromatin structure facilitate changes in gene expression needed to specify distinct cell types. To understand how changes in chromatin structure influence the developmental trajectory of neural progenitor cells, we developed CUT&TIME, a technique that uses a hyperactive ...
Eldred, K. C.
•
Wooten, M.
•
Janssens, D. H.
•
Hahn, J.
...•
Henikoff, S.
bioRxiv
Sun Sep 07 2025
Human-mouse cross-species comparison identifies common and unique aspects of intestinal mesenchyme development
Using single-cell RNA sequencing (scRNA-seq) and histological approaches, we examine cross-species cellular identity, diversity and organization of mesenchymal (fibroblast) populations in the developing mouse and human intestines. In both species, we defined 7 fibroblast populations. Using cross-species integration and label transfer approaches we find that each mesenchymal cell subtype in the mur...
Johnson, K.
•
Dong, X.
•
Xiao, Z.
•
Islam, H.
...•
Walton, K. D.
bioRxiv
Sun Sep 07 2025
Bruce suppresses autophagy-regulated caspase activity and wing tissue growth in Drosophila
Caspases are cysteine-aspartic proteases that mediate both lethal and non-lethal cellular outcomes, including the promotion of tissue growth. However, the mechanisms underlying the differential regulation of these activities remain unclear. We have previously shown that among the two Drosophila executioner caspases, Dcp-1 and Drice, Dcp-1 promotes tissue growth in a non-lethal manner, independent ...
Shinoda, N.
•
Hama, Y.
•
Hanawa, N.
•
Miura, M.
bioRxiv
Sat Sep 06 2025
Apical extracellular matrix regulates fold morphogenesis in the Drosophila wing disc
Tissue folding is a fundamental process occurring often in animal organ development. Here, we study the progression of fold shape and the underlying mechanics in the development of the Drosophila wing disc. We present a 3D segmentation of the apical surface of the wing disc proper from larval stages, when folds grow, to early pupariation, when the tissue unfolds and remodels into a bilayer. We est...
Fuhrmann, J. F.
•
Schimmenti, V. M.
•
Cwikla, G.
•
Lee, S.
...•
Dye, N. A.
bioRxiv
Sat Sep 06 2025
Single-cell mitochondrial lineage tracing decodes fate decision and spatial clonal architecture in human hematopoietic organoids
The ability to perform lineage tracing at the single-cell level is critical to reconstructing dynamic transitions during cell differentiation. However, prospective tracing approaches inevitably encounter outstanding challenges including barcoding precision, barcode diversity, and detection efficiency, which can skew inferred lineage relationships. Human pluripotent stem cells (hPSCs) even face ris...
Xue, Y.
•
Su, J.
•
Chao, Y.
•
Liu, L.
...•
Sugimura, R.
bioRxiv
Sat Sep 06 2025
Spatiotemporal proteomics reveals dynamic antagonistic gradients shaping signalling waves
Embryonic development is driven by dynamic protein networks, yet how these dynamics shape morphogenesis remains incompletely understood. Somitogenesis, the rhythmic segmentation of vertebrate embryos, is governed by signalling gradients and oscillations in the presomitic mesoderm (PSM), but the corresponding protein dynamics are largely unknown. Perturbations in this process cause congenital spine...
Meijer, W.
•
Ribeiro de Andrade, V.
•
Stelloo, S.
•
Thomas, W.
...•
Sonnen, I.