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September 9th, 2025
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Yale University
neuroscience
bioRxiv

Human-specific features of the cerebellum and ZP2-regulated synapse development

Kim, S.-K.Open in Google Scholar•Cherskov, A.Open in Google Scholar•Sindhwani, A.Open in Google Scholar•Choi, S.-H.Open in Google Scholar•Kim, H.Open in Google Scholar•Li, M.-L.Open in Google Scholar•Zhang, M.Open in Google Scholar•Mato-Blanco, X.Open in Google Scholar•Liu, Y.Open in Google Scholar•Micali, N.Open in Google Scholaret al.

Understanding the unique features of the human brain compared to non-human primates has long intrigued humankind. The cerebellum refines motor coordination and cognitive functions, contributing to the evolutionary development of human adaptability and dexterity. To identify shared and divergent features across primates, we conducted single-nucleus transcriptomic and chromatin accessibility profiling of the adult cerebellar cortex in humans, chimpanzees, macaques, and marmosets. We revealed human-specific transcriptomic and regulatory features, particularly those involved in synaptogenesis. Notably, we identified an enrichment of the sperm receptor zona pellucida glycoprotein 2 (ZP2) and its potential interactors, known for their roles in gamete interaction, in human granule cells. Experimental data show that ZP2 expression in human granule cells is induced by pontine mossy fibers, reducing synaptic proteins at pontocerebellar glomerular synapses, and decreasing cerebellar neuron electrophysiological activity. This unexpected co-option of ZP2 in human-specific synapse regulation provides insights into the evolutionary specialization of the human cerebellum.

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