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September 9th, 2025
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CASIA
neuroscience
bioRxiv

PIB: Parallel ion beam etching of sections collected on wafer for ultra large-scale connectomics

Ma, H.Open in Google Scholar•Lv, Y.Open in Google Scholar•Wang, M.Open in Google Scholar•Zhao, Z.Open in Google Scholar•Liu, J.Open in Google Scholar•Dong, X.Open in Google Scholar•Zhang, Y.Open in Google Scholar•Guo, J.Open in Google Scholar•Chen, B.Open in Google Scholar•Zhang, L.Open in Google Scholaret al.

We have developed a parallel ion beam etching device enabling simultaneous 20 nm thickness reduction for hundreds of biological sections, which could be collected on a whole 4-inch wafer. Through cyclic scanning electron microscopy imaging and ion beam thinning, it is trustworthy and efficient to obtain three-dimensional volume with high axial resolution for millimeter-size samples compared to conventional volume electron microscopy methods.

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