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September 9th, 2025
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University of the Basque Country (UPV/EHU)
neuroscience
bioRxiv

Characterization of the Lipidome of Neurons in Mouse Brain Nuclei using Imaging Mass Spectrometry

Huergo, C.Open in Google Scholar•De las Heras-Garcia, L.Open in Google Scholar•Razquin, J.Open in Google Scholar•Rueda, Y.Open in Google Scholar•Miguelez, C.Open in Google Scholar•Fernandez, J. A.Open in Google Scholar

Understanding the molecular composition of the brain at cellular level is essential for deciphering the metabolic alterations associated with brain diseases. Furthermore, the different prevalence of some neurological diseases between males and fe-males highlight the importance of incorporating gender factor in such studies. Here, we demonstrate that using imaging mass spectrometry in negative polarity it is possible to isolate and characterize the lipidome of specific neuronal populations in the mouse brain, including the locus coeruleus (LC), mesencephalic neurons and the substantia nigra pars compacta (SNc). Neuronal identity was validated through immunofluorescence on adjacent serial sections. Comparative analysis revealed that each neuronal population presents a distinct and well-defined lipidic profile, with differences extending across all lipid classes analyzed. Regarding sex-based differences, we found discrete differences in phosphatidylcholine/phosphatidylethanolamine-ether, phosphatidylinositol and sphingomyelin LC neurons. Lipidomic differences were more pronounced in mesencephalic neurons, whereas no significant sex-defendant differences were observed in SNc lipid composition. These findings lay the groundwork for future studies aimed at identifying lipid metabolic dysregulations in the context of neurodegenerative diseases.

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