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April 11th, 2025
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Department of Chemistry - BMC, Uppsala University; Center of Excellence for the Chemical Mechanisms of Life, Uppsala University
neuroscience
biorxiv

Next-level high-precision spatial omics enabled through spatial similarity networking

Sharma, V. V.Open in Google Scholar•Toth, G.Open in Google Scholar•Martinis, R.Open in Google Scholar•Hansen, C. E.Open in Google Scholar•Kooij, G.Open in Google Scholar•Lanekoff, I.Open in Google Scholar

Unambiguous molecular annotations are essential to discern complex local biochemical processes in spatial biology. Here, we acquire conventional mass spectrometry images (MSI) in parallel with hundreds of tandem MSI and introduce spatial similarity networks as an additional dimension for molecular annotation. Overall, our approach enables the deconvolution and annotation of superimposed and otherwise unresolvable molecular images, advancing spatial omics to the frontier of comprehensive omics technologies.

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